| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UCP2 |
| Uniprot No | P55851 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MVGFKATDVPPTATVKFLGAGTAACIADLITFPLGTAKVRLQIQGESQGP VRATASAQYRGVMGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLY DSVKQFYTKGSEHASIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARA GGGRRYQSTVNAYKTIAREEGFRGLWKGTSPNVARNAIVNCAELVTYDLI KDALLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQY SSAGHCALTMLQKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMA ACTSREAPF |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于UCP2重组蛋白的参考文献(示例为假设性文献，仅供参考)：
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1. **文献名称**：*Recombinant UCP2 expression and its role in mitochondrial proton leak*
**作者**：Zhang Y, et al.
**摘要**：研究通过大肠杆菌系统成功表达重组UCP2蛋白，并验证其在线粒体质子泄漏中的功能，揭示了UCP2在能量代谢调节中的关键作用。
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2. **文献名称**：*Purification and functional characterization of human UCP2 recombinant protein*
**作者**：Esteves TC, et al.
**摘要**：利用哺乳动物细胞表达系统纯化重组人源UCP2蛋白，发现其在抑制活性氧(ROS)生成中的作用，为UCP2的抗氧化机制提供实验依据。
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3. **文献名称**：*Structural insights into UCP2-mediated lipid transport using recombinant protein models*
**作者**：Alvarez-Suarez D, et al.
**摘要**：通过重组UCP2蛋白的结晶和结构解析，阐明其与脂肪酸结合的结构域，提出UCP2可能通过脂质转运调节线粒体膜电位的假说。
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4. **文献名称**：*Development of a novel assay for UCP2 activity using recombinant protein*
**作者**：Bouillaud F, et al.
**摘要**：建立基于重组UCP2蛋白的体外活性检测方法，证明其在质子转运中的pH依赖特性，为药物筛选提供技术平台。
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(注：以上文献为示例，实际引用需以真实发表的论文为准。)
**Background of UCP2 Recombinant Protein**
Uncoupling Protein 2 (UCP2) is a mitochondrial transmembrane protein belonging to the solute carrier family, primarily involved in regulating energy metabolism and oxidative stress. Unlike UCP1. which is specific to brown adipose tissue and mediates thermogenesis, UCP2 is ubiquitously expressed in tissues such as the brain, pancreas, and immune cells. It modulates proton leakage across the mitochondrial inner membrane, uncoupling oxidative phosphorylation from ATP synthesis, thereby reducing ATP production and reactive oxygen species (ROS) generation. This unique function links UCP2 to metabolic homeostasis, immune responses, and cellular protection against oxidative damage.
Recombinant UCP2 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to study its structure, function, and therapeutic potential. Researchers produce UCP2 by inserting its gene into expression vectors, followed by transfection into host cells, purification via affinity chromatography (e.g., His-tag), and validation using techniques like Western blot or mass spectrometry. Recombinant UCP2 retains biological activity, enabling *in vitro* and *in vivo* studies on its role in metabolic disorders (e.g., obesity, diabetes), cancer (modulating apoptosis and proliferation), and neurodegenerative diseases (e.g., Alzheimer’s, via ROS regulation).
Interest in UCP2 also stems from its dual role in health and disease. While its antioxidant properties are cytoprotective, overexpression may contribute to metabolic inefficiency or tumor progression. Recombinant UCP2 facilitates drug screening targeting mitochondrial dysfunction and serves as a tool to explore UCP2-specific mechanisms. Challenges remain in ensuring proper protein folding, post-translational modifications, and mitochondrial localization *in vitro*. Nonetheless, recombinant UCP2 remains pivotal for advancing therapeutic strategies in metabolic and age-related diseases.
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