| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFM1 |
| Uniprot No | Q96RP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-751aa |
| 氨基酸序列 | SSSGV IPNEKIRNIG ISAHIDSGKT TLTERVLYYT GRIAKMHEVK GKDGVGAVMD SMELERQRGI TIQSAATYTM WKDVNINIID TPGHVDFTIE VERALRVLDG AVLVLCAVGG VQCQTMTVNR QMKRYNVPFL TFINKLDRMG SNPARALQQM RSKLNHNAAF MQIPMGLEGN FKGIVDLIEE RAIYFDGDFG QIVRYGEIPA ELRAAATDHR QELIECVANS DEQLGEMFLE EKIPSISDLK LAIRRATLKR SFTPVFLGSA LKNKGVQPLL DAVLEYLPNP SEVQNYAILN KEDDSKEKTK ILMNSSRDNS HPFVGLAFKL EVGRFGQLTY VRSYQGELKK GDTIYNTRTR KKVRLQRLAR MHADMMEDVE EVYAGDICAL FGIDCASGDT FTDKANSGLS MESIHVPDPV ISIAMKPSNK NDLEKFSKGI GRFTREDPTF KVYFDTENKE TVISGMGELH LEIYAQRLER EYGCPCITGK PKVAFRETIT APVPFDFTHK KQSGGAGQYG KVIGVLEPLD PEDYTKLEFS DETFGSNIPK QFVPAVEKGF LDACEKGPLS GHKLSGLRFV LQDGAHHMVD SNEISFIRAG EGALKQALAN ATLCILEPIM AVEVVAPNEF QGQVIAGINR RHGVITGQDG VEDYFTLYAD VPLNDMFGYS TELRSCTEGK GEYTMEYSRY QPCLPSTQED VINKYLEATG QLPVKKGKAK N |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GFM1重组蛋白研究的虚构参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **文献名称**：Recombinant expression and functional characterization of human GFM1 in Escherichia coli
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人源GFM1蛋白，验证了其GTP酶活性及其在线粒体翻译延伸过程中的作用，为结构-功能研究提供了工具。
2. **文献名称**：Crystal structure analysis of GFM1 reveals insights into mitochondrial translation mechanisms
**作者**：Tanaka K, et al.
**摘要**：通过重组表达获得高纯度GFM1蛋白并解析其晶体结构，揭示了其与线粒体核糖体相互作用的分子基础，为遗传性线粒体疾病相关突变研究提供依据。
3. **文献名称**：Functional rescue of GFM1 mutations by recombinant protein supplementation in cell models
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞系统表达重组GFM1蛋白，证明外源性补充功能性GFM1可部分恢复突变细胞模型的线粒体翻译缺陷，提示潜在治疗策略。
4. **文献名称**：Optimization of GFM1 recombinant production in yeast for high-throughput screening
**作者**：Müller J, et al.
**摘要**：开发了基于酵母系统的GFM1重组表达平台，优化后的蛋白产量满足药物筛选需求，并用于筛选靶向线粒体翻译的小分子调节剂。
**注意**：以上为模拟文献示例，真实文献需通过PubMed/Google Scholar检索关键词如"GFM1 recombinant expression"或"GFM1 mitochondrial translation"获取。GFM1相关研究多集中于其在线粒体蛋白质合成中的功能及突变致病机制。
**Background of GFM1 Recombinant Protein**
GFM1 (G-domain of elongation factor-G 1), also known as mitochondrial elongation factor G1 (EF-G1mt), is a critical protein involved in mitochondrial translation. It functions as a GTPase that catalyzes the translocation of transfer RNA (tRNA) and messenger RNA (mRNA) during the elongation phase of mitochondrial protein synthesis. This process is essential for the production of oxidative phosphorylation (OXPHOS) complex subunits, which are required for ATP generation in eukaryotic cells.
The GFM1 gene encodes a 751-amino-acid protein localized to the mitochondrial matrix. Structurally, GFM1 contains conserved GTP-binding domains and a C-terminal region that interacts with the mitochondrial ribosome. Mutations in GFM1 are linked to severe mitochondrial disorders, such as combined oxidative phosphorylation deficiency 1 (COXPD1), characterized by neurological dysfunction, muscle weakness, and early lethality. These clinical associations underscore its non-redundant role in mitochondrial homeostasis.
Recombinant GFM1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its purification often involves affinity chromatography tags (e.g., His-tag) followed by biochemical characterization, including GTPase activity assays and ribosome-binding studies. Researchers utilize recombinant GFM1 to investigate mitochondrial translation mechanisms, model disease-associated mutations *in vitro*, and screen for therapeutic compounds targeting mitochondrial dysfunction.
Current research on GFM1 recombinant protein also explores its interaction with antibiotics or small molecules that modulate mitochondrial translation, offering potential avenues for treating mitochondrial diseases or enhancing cellular energy metabolism. Understanding GFM1's molecular dynamics remains pivotal for deciphering mitochondrial pathologies and developing targeted therapies.
×
