| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPAM |
| Uniprot No | Q9HCL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-828aa |
| 氨基酸序列 | MDESALTLGTIDVSYLPHSSEYSVGRCKHTSEEWGECGFRPTIFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWDKFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYVLFIQERDVHKGMFATNVTENVLNSSRVQEAIAEVAAELNPDGSAQQQSKAVNKVKKKAKRILQEMVATVSPAMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDVLYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSTNVIPDILIIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGCVRVDFAQPFSLKEYLESQSQKPVSALLSLEQALLPAILPSRPSDAADEGRDTSINESRNATDESLRRRLIANLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIDLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRNDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSLYAVLNKRGLGGPTSTPPNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEHDDQEDISPSLAEQQWDKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVHNFSGPVPEPEYLQKLHKYLITRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRVSVLELSSTFLPQCNRQKLLEYILSFVVL |
| 预测分子量 | 93,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPAM(甘油-3-磷酸酰基转移酶)重组蛋白的3篇代表性文献摘要，涵盖结构、功能及表达研究：
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1. **文献名称**：*"Crystal structure of the human mitochondrial glycerol-3-phosphate acyltransferase 1"*
**作者**：J. Liu et al.
**摘要**：通过昆虫细胞系统重组表达了人源GPAM的催化结构域，并利用X射线晶体学解析了其2.8Å分辨率的结构。研究发现其活性位点由疏水口袋和关键氨基酸残基(如His-342)组成，揭示了底物结合与酰基转移的分子机制。
2. **文献名称**：*"Functional characterization of recombinant murine GPAT1 in lipid metabolism"*
**作者**：S. Takeuchi et al.
**摘要**：在大肠杆菌中成功表达并纯化小鼠GPAM重组蛋白，体外酶活实验表明其特异性催化棕榈酰-CoA与甘油-3-磷酸结合。研究还发现GPAM活性受磷酸化调控，提示其在肝脏脂质合成中的关键作用。
3. **文献名称**：*"Development of a high-throughput assay for GPAM activity using recombinant protein"*
**作者**：K. R. Vallurupalli et al.
**摘要**：通过哺乳动物细胞表达系统获得高纯度GPAM重组蛋白，建立基于荧光底物的高通量筛选平台，用于发现小分子抑制剂。该研究为靶向GPAM的抗肥胖药物开发提供了技术基础。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以关键词“GPAM recombinant”“GPAT1 expression”检索最新论文。部分研究可能涉及疾病模型(如非酒精性脂肪肝)或代谢调控机制。
**Background of GPAM Recombinant Protein**
Glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), is a key enzyme in lipid metabolism, catalyzing the initial step of glycerolipid biosynthesis. It transfers an acyl group from acyl-CoA to glycerol-3-phosphate, producing lysophosphatidic acid (LPA), a precursor for triglycerides and phospholipids. GPAM is localized to the mitochondrial outer membrane and plays a critical role in energy storage, membrane formation, and lipid signaling. Dysregulation of GPAM activity is linked to metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD), making it a potential therapeutic target.
Recombinant GPAM proteins are engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), these proteins retain enzymatic activity and structural integrity, enabling mechanistic studies, inhibitor screening, and structural analysis (e.g., X-ray crystallography). Recombinant GPAM is often tagged (e.g., His-tag) for purification and detection, and its activity is assessed using spectrophotometric or fluorometric assays.
Recent studies focus on developing GPAM inhibitors to modulate lipid synthesis, with potential applications in treating metabolic syndromes. Additionally, recombinant GPAM aids in understanding isoform-specific functions, as mammals express multiple GPAT isoforms with distinct roles. Challenges include maintaining protein stability and mimicking native post-translational modifications *in vitro*.
Overall, GPAM recombinant protein serves as a vital tool for unraveling lipid metabolism pathways and advancing targeted therapies for metabolic diseases.
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