| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNY |
| Uniprot No | Q8ND76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-341aa |
| 氨基酸序列 | GNTTSCCVS SSPKLRRNAH SRLESYRPDT DLSREDTGCN LQHISDRENI DDLNMEFNPS DHPRASTIFL SKSQTDVREK RKSLFINHHP PGQIARKYSS CSTIFLDDST VSQPNLKYTI KCVALAIYYH IKNRDPDGRM LLDIFDENLH PLSKSEVPPD YDKHNPEQKQ IYRFVRTLFS AAQLTAECAI VTLVYLERLL TYAEIDICPA NWKRIVLGAI LLASKVWDDQ AVWNVDYCQI LKDITVEDMN ELERQFLELL QFNINVPSSV YAKYYFDLRS LAEANNLSFP LEPLSRERAH KLEAISRLCE DKYKDLRRSA RKRSASADNL TLPRWSPAII S |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNY(Cyclin Y)重组蛋白研究的示例参考文献(注：以下内容为模拟虚构，仅供参考检索方向)：
---
1. **文献名称**: *"Cyclin Y phosphorylation of LRP6 regulates Wnt/β-catenin signaling"*
**作者**: Zhang, L., et al.
**摘要**: 本研究利用重组CCNY蛋白，揭示了其通过磷酸化LRP6受体增强Wnt/β-catenin信号通路的分子机制，证实CCNY在胚胎发育和肿瘤发生中的调控作用。
2. **文献名称**: *"Structural basis of Cyclin Y-CDK14 kinase complex assembly"*
**作者**: Thompson, R., & Chen, H.
**摘要**: 通过重组表达CCNY与CDK14蛋白，解析了二者复合物的晶体结构，阐明了其相互作用界面及对细胞周期G1/S期转换的影响。
3. **文献名称**: *"Recombinant CCNY promotes glioblastoma invasion via MMP-9 activation"*
**作者**: Gupta, S., et al.
**摘要**: 实验表明，重组CCNY蛋白通过激活基质金属蛋白酶MMP-9.促进胶质母细胞瘤细胞的迁移和侵袭，提示其作为潜在治疗靶点。
4. **文献名称**: *"CCNY interacts with PP2A/B55α to regulate cell cycle progression"*
**作者**: Watanabe, K., et al.
**摘要**: 利用重组蛋白技术，发现CCNY通过结合磷酸酶PP2A/B55α亚基调控有丝分裂进程，为细胞周期检查点机制提供新见解。
---
**检索建议**：如需真实文献，可在PubMed或Google Scholar搜索关键词：
`"Cyclin Y recombinant protein"`、`"CCNY cloning"`、`"CCNY Wnt signaling"`、`"CCNY cell cycle"`。
**Background of CCNY Recombinant Protein**
CCNY (Cell Cycle Protein Y) is a regulatory protein involved in cell cycle progression and signaling pathways. It belongs to the cyclin family, which plays a critical role in coordinating transitions between cell cycle phases by activating cyclin-dependent kinases (CDKs). Unlike classical cyclins, CCNY lacks the conserved cyclin box domain but contains a unique N-terminal region that facilitates interactions with CDK14 and CDK16. This interaction is essential for modulating Wnt signaling, a pathway crucial for embryonic development, tissue homeostasis, and cancer progression.
Recombinant CCNY protein is engineered through genetic cloning, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables researchers to study CCNY's structure-function relationships, kinase activation mechanisms, and its role in diseases. Studies have linked CCNY overexpression to various cancers, including lung, breast, and liver cancers, where it promotes cell proliferation and metastasis by enhancing Wnt/β-catenin signaling.
Additionally, CCNY recombinant protein is utilized in drug discovery to screen inhibitors targeting the CCNY-CDK14/16 axis, offering potential therapeutic strategies. Its application extends to elucidating crosstalk between cell cycle regulation and developmental pathways, such as Hedgehog and Notch. Despite progress, the precise molecular mechanisms of CCNY in non-canonical signaling contexts remain under investigation.
In summary, CCNY recombinant protein serves as a vital tool for dissecting its biological roles and advancing translational research in oncology and developmental biology.
×
