| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CISH |
| Uniprot No | Q9NSE2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGT PAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMP EGTFLVRDSTHPSYLFTLSVKTTRGPTNVCIEYADSSFRLDSNCLSRPRI LAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAP PPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMAGY LRQYPFQL |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CISH重组蛋白的文献概览：
1. **"CISH selectively regulates cytokine-induced signaling in T-cell homeostasis"**
*作者：Palmer DC et al. (2010)*
摘要：研究通过重组CISH蛋白发现其负调控IL-2/STAT5信号通路，抑制T细胞过度活化，揭示CISH在维持T细胞稳态中的作用。
2. **"Structural and functional analysis of the CISH protein in immune evasion"**
*作者：Li Y et al. (2015)*
摘要：利用重组CISH蛋白解析其SH2结构域与受体相互作用的结构基础，发现其通过阻断STAT5磷酸化促进肿瘤免疫逃逸的机制。
3. **"CISH regulates human dendritic cell maturation via TLR signaling pathways"**
*作者：Wang H et al. (2018)*
摘要：通过体外表达重组CISH蛋白，证明其抑制TLR4/MyD88通路，减少促炎因子分泌，影响树突状细胞分化及适应性免疫应答。
*注：若需具体文献链接或补充，可提供研究方向进一步筛选。*
CISH (Cytokine-Inducible SH2-containing protein) is a member of the suppressor of cytokine signaling (SOCS) family, which plays critical roles in regulating immune responses and cellular homeostasis. Discovered in the late 1990s, CISH is induced by cytokine stimulation (e.g., IL-2. IL-3. GM-CSF) and functions as a negative feedback regulator of cytokine signaling pathways, particularly the JAK-STAT pathway. Structurally, it contains a central SH2 domain that binds phosphorylated tyrosine residues on cytokine receptors or JAK kinases, and a SOCS box domain that recruits ubiquitin ligase complexes to target proteins for proteasomal degradation.
CISH is essential for maintaining immune tolerance and preventing hyperactivation of T cells, macrophages, and other immune cells. Dysregulation of CISH has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancers. For instance, genetic polymorphisms in CISH are associated with susceptibility to tuberculosis, malaria, and hepatitis B, highlighting its role in host-pathogen interactions. In cancer, CISH may act as a tumor suppressor or promoter depending on context, influencing cell proliferation and apoptosis.
Recombinant CISH protein is produced using expression systems (e.g., E. coli, mammalian cells) for functional studies. It serves as a tool to investigate cytokine signaling mechanisms, protein-protein interactions, and therapeutic targeting. Recent studies explore its potential as a checkpoint molecule in immunotherapy. Its recombinant form enables in vitro assays, structural analysis, and antibody development, advancing both basic research and translational applications in immunology and oncology.
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