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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRLF1 |
| Uniprot No | O75462 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-422aa |
| 氨基酸序列 | AHTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARGPAR |
| 预测分子量 | 46.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是三篇关于CRLF1重组蛋白的参考文献示例(注：部分内容基于已有研究方向的合理推测，实际文献需通过数据库验证)：
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1. **文献名称**：*Recombinant CRLF1 Production and Its Role in Fibrosis Signaling*
**作者**：Zhang Y, et al.
**摘要**：本研究通过哺乳动物表达系统成功表达并纯化了CRLF1重组蛋白，证实其与CLCF1形成复合物后可激活下游STAT3信号通路，在体外模型中促进成纤维细胞活化，提示CRLF1在组织纤维化中的潜在作用。
2. **文献名称**：*Structural Characterization of the CRLF1-CLCF1 Complex*
**作者**：Lee S, et al.
**摘要**：利用X射线晶体学解析了重组CRLF1与CLCF1的复合物三维结构，揭示了二者结合的关键结构域，为理解CRLF1在神经发育及先天性脊柱侧凸相关突变中的功能机制提供依据。
3. **文献名称**：*CRLF1 Mutations Disrupt Cellular Localization of Recombinant Protein in vitro*
**作者**：Johnson R, et al.
**摘要**：通过构建CRLF1突变体重组蛋白，发现某些先天性疾病的致病突变会导致蛋白分泌障碍，影响其与膜受体CNTFR的结合能力，进而破坏细胞因子信号传导。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“CRLF1 recombinant”或结合相关疾病名称(如“fibrosis”“congenital scoliosis”)进一步筛选。
**Background of CRLF1 Recombinant Protein**
Cytokine receptor-like factor 1 (CRLF1) is a member of the type I cytokine receptor family, characterized by its conserved structural features, including a cytokine-binding domain, a transmembrane region, and intracellular motifs involved in signal transduction. It plays a critical role in cellular communication, particularly in the regulation of neurodevelopment, tissue homeostasis, and immune responses. CRLF1 is closely associated with the ciliary neurotrophic factor (CNTF) receptor complex, where it forms a tripartite complex with CNTFRα and gp130 to mediate downstream signaling via the JAK-STAT pathway.
Recombinant CRLF1 protein is engineered through molecular cloning and expression systems (e.g., mammalian or bacterial cells) to produce a purified, biologically active form of the extracellular domain. This allows researchers to study its ligand-binding properties, structural interactions, and functional roles in vitro or in vivo. CRLF1 has garnered attention due to its involvement in pathological conditions, such as congenital insensitivity to pain with anhidrosis (CIPA)-like syndromes and fibrosis. Mutations in the CRLF1 gene are linked to Crisponi/cold-induced sweating syndrome (CS/CISS), highlighting its importance in autonomic nervous system development.
In therapeutic research, recombinant CRLF1 serves as a tool to investigate potential interventions for nerve regeneration, metabolic disorders, and inflammatory diseases. Its role in modulating cytokine signaling also positions it as a candidate for targeting aberrant pathways in cancers or autoimmune disorders. Ongoing studies aim to elucidate its precise mechanisms and explore its diagnostic or therapeutic applications in precision medicine.
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