| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP7A1 |
| Uniprot No | P22680 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL |
| 预测分子量 | 61.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP7A1重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**：*"Expression and Purification of Human Cholesterol 7α-Hydroxylase in a Baculovirus System"*
**作者**：Li et al.
**摘要**：该研究利用杆状病毒-昆虫细胞系统成功表达了功能性人源CYP7A1重组蛋白，并通过亲和层析纯化，验证了其催化胆固醇生成7α-羟基胆固醇的活性，为体外研究胆汁酸合成机制提供了工具。
2. **文献名称**：*"Functional Characterization of Recombinant CYP7A1 in Lipid Metabolism Using a Yeast Expression Model"*
**作者**：Chiang et al.
**摘要**：作者在酵母中异源表达CYP7A1.证明重组蛋白可调控宿主细胞的固醇代谢通路，并通过突变实验揭示了酶活性位点对底物结合的关键作用，为代谢疾病相关药物筛选提供了模型。
3. **文献名称**：*"Structural Insights into CYP7A1 via Cryo-EM: Implications for Bile Acid Biosynthesis"*
**作者**：Zhang et al.
**摘要**：该研究通过冷冻电镜解析了重组CYP7A1蛋白的高分辨率结构，阐明了其与细胞色素P450还原酶的相互作用机制，并揭示了底物通道的构象变化，为靶向CYP7A1的药物设计提供了结构基础。
注：以上文献信息为示例性质，具体内容可能需要根据实际发表文献调整。建议通过PubMed或Web of Science以“CYP7A1 recombinant expression”为关键词检索最新研究。
CYP7A1 (cholesterol 7α-hydroxylase) is a cytochrome P450 enzyme central to cholesterol homeostasis and bile acid biosynthesis. Located in the endoplasmic reticulum of hepatocytes, it catalyzes the rate-limiting step in the classical pathway of bile acid synthesis, converting cholesterol into 7α-hydroxycholesterol. This enzymatic reaction not only regulates cholesterol catabolism but also influences systemic lipid metabolism, making CYP7A1 a critical mediator of metabolic balance. Its activity is tightly controlled by transcriptional regulators (e.g., FXR, SREBP) and post-translational mechanisms to prevent cholesterol accumulation or deficiency.
Recombinant CYP7A1 protein is produced using heterologous expression systems, such as Escherichia coli, yeast, or mammalian cell lines, enabling large-scale purification for functional studies. The engineered protein retains catalytic activity and structural features of the native enzyme, including its membrane-binding domain and heme-cofactor binding site. Researchers utilize recombinant CYP7A1 to investigate enzyme kinetics, substrate specificity, and interactions with regulatory molecules. It also serves as a tool for drug discovery, particularly for metabolic disorders like hypercholesterolemia, non-alcoholic fatty liver disease (NAFLD), and gallstone formation. Dysregulation of CYP7A1 expression is linked to altered bile acid profiles and lipid abnormalities, underscoring its therapeutic relevance. Studies involving recombinant protein have elucidated mechanisms of genetic polymorphisms and environmental factors (e.g., diets, drugs) affecting CYP7A1 function, advancing precision medicine approaches for cholesterol-related diseases.
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