| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP5A1 |
| Uniprot No | P24557 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-533aa |
| 氨基酸序列 | MEALGFLKLEVNGPMVTVALSVALLALLKWYSTSAFSRLEKLGLRHPKPSPFIGNLTFFRQGFWESQMELRKLYGPLCGYYLGRRMFIVISEPDMIKQVLVENFSNFTNRMASGLEFKSVADSVLFLRDKRWEEVRGALMSAFSPEKLNEMVPLISQACDLLLAHLKRYAESGDAFDIQRCYCNYTTDVVASVAFGTPVDSWQAPEDPFVKHCKRFFEFCIPRPILVLLLSFPSIMVPLARILPNKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQDFDIVRDVFSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATYLLATNPDCQEKLLREVDVFKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAAQDCEVLGQRIPAGAVLEMAVGALHHDPEHWPSPETFNPERFTAEARQQHRPFTYLPFGAGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQLESKSALGPKNGVYIKIVSR |
| 预测分子量 | 60,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP5A1重组蛋白的假设性参考文献示例(注：CYP5A1可能为笔误或非标准命名，建议核实基因/蛋白名称。以下内容仅供示例参考)：
1. **文献名称**：*Heterologous Expression and Functional Characterization of Recombinant CYP5A1 in E. coli*
**作者**：Smith J, et al.
**摘要**：本研究成功将CYP5A1基因克隆至大肠杆菌表达系统，纯化获得活性重组蛋白，并证实其在体外对特定甾体底物的催化活性，为后续代谢研究奠定基础。
2. **文献名称**：*Structural Insights into CYP5A1 through Recombinant Protein Crystallography*
**作者**：Lee H, et al.
**摘要**：利用昆虫细胞表达系统制备CYP5A1重组蛋白，解析其三维结构，揭示底物结合口袋的关键氨基酸残基，探讨其与酶特异性的关系。
3. **文献名称**：*Role of Recombinant CYP5A1 in Drug Metabolism Screening*
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物HEK293细胞表达CYP5A1重组蛋白，结合体外代谢实验，证明其对某类抗癌药物的羟基化代谢作用，提示其潜在药理学意义。
**建议**：
- 若检索困难，可能需确认目标蛋白是否为**CYP51A1**(固醇合成酶)或**CYP3A4**等常见P450酶，或检查物种特异性命名(如植物/微生物来源)。
- 推荐使用PubMed或UniProt数据库核实蛋白名称及最新文献。
Here’s a concise overview of recombinant CYP5A1 protein:
CYP5A1 (Cytochrome P450 Family 5 Subfamily A Member 1) is a member of the cytochrome P450 enzyme superfamily, which plays critical roles in oxidative metabolism of endogenous and exogenous compounds, including drugs, toxins, and lipids. While less characterized than other CYP isoforms like CYP3A4 or CYP2D6. CYP5A1 is hypothesized to contribute to lipid metabolism, particularly in pathways involving arachidonic acid and eicosanoid biosynthesis. Its exact physiological substrates and tissue-specific functions remain under investigation, though studies suggest potential links to vascular and inflammatory processes.
Recombinant CYP5A1 protein is produced using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells) to enable functional and structural studies. These systems often incorporate modifications such as N-terminal truncations or fusion tags (e.g., His-tags) to enhance solubility and simplify purification. The recombinant protein allows researchers to study CYP5A1’s catalytic activity, substrate specificity, and interactions with inhibitors or regulatory proteins in controlled settings. Applications include drug metabolism profiling, enzyme kinetic analyses, and structural biology (e.g., crystallography or cryo-EM) to elucidate substrate-binding mechanisms. Challenges in working with recombinant CYP5A1 mirror those of membrane-bound CYPs, requiring reconstitution with lipids or nanodiscs to maintain native conformation. Current research focuses on clarifying its biological role and potential as a therapeutic target in lipid-related disorders.
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