| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP26B1 |
| Uniprot No | Q9NR63 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | MLFEGLDLVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQGSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVSNSIGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRRGIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIFAAYATTASASTSLIMQLLKHPTVLEKLRDELRAHGILHSGGCPCEGTLRLDTLSGLRYLDCVIKEVMRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDPDRFSQARSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRITLVPVLHPVDGLSVKFFGLDSNQNEILPETEAMLSATV |
| 预测分子量 | 63.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP26B1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**：*"Expression and Characterization of Recombinant Human CYP26B1"*
**作者**：Tahayato et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统成功表达并纯化了重组人CYP26B1蛋白。通过体外酶活实验证实其能高效催化全反式视黄酸(ATRA)的羟基化代谢，并发现其活性受细胞色素P450还原酶(CPR)的调控。该研究为CYP26B1的酶动力学及抑制剂筛选提供了工具。
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2. **文献名称**：*"Structural Insights into CYP26B1 Inhibition by Novel Small Molecules"*
**作者**：Lees et al.
**摘要**：通过大肠杆菌表达系统制备重组CYP26B1蛋白，结合X射线晶体学解析其三维结构，并筛选出新型小分子抑制剂。研究揭示了关键活性位点残基与抑制剂的相互作用机制，为开发靶向CYP26B1的疾病治疗药物(如银屑病)奠定基础。
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3. **文献名称**：*"Functional Analysis of CYP26B1 in Retinoic Acid Homeostasis During Zebrafish Development"*
**作者**：White et al.
**摘要**：利用重组CYP26B1蛋白进行体外代谢实验，结合斑马鱼模型验证其调控视黄酸浓度的功能。结果表明，CYP26B1通过降解ATRA影响胚胎神经嵴细胞分化，强调其在发育中的关键作用及重组蛋白在功能研究中的实用性。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对具体信息。若需扩展，可搜索关键词“CYP26B1 recombinant expression”或“CYP26B1 enzymatic assay”获取更多研究。
CYP26B1 is a member of the cytochrome P450 superfamily, specifically classified under the CYP26 subfamily, which plays a critical role in the metabolism of retinoic acid (RA), an active derivative of vitamin A. As a key regulatory enzyme, CYP26B1 catalyzes the hydroxylation of RA into inactive metabolites, thereby tightly controlling its intracellular concentration. This precise regulation is vital for maintaining cellular homeostasis, as RA acts as a potent signaling molecule involved in embryonic development, cellular differentiation, and tissue morphogenesis. Dysregulation of CYP26B1 has been linked to developmental abnormalities, skin disorders, and cancer, highlighting its physiological and pathological significance.
Recombinant CYP26B1 protein is produced using biotechnological methods, typically through heterologous expression in systems like bacterial (E. coli), yeast, or mammalian cell lines. The recombinant form retains the enzymatic activity of the native protein, enabling researchers to study its structure, substrate specificity, and interaction with inhibitors or modulators in vitro. Its production often involves codon optimization, affinity tag fusion (e.g., His-tag), and purification via chromatography to ensure high purity and functionality.
Research applications of recombinant CYP26B1 span drug discovery and toxicology. It is used to screen potential inhibitors targeting RA metabolism, which could have therapeutic value in cancers or dermatological conditions driven by RA imbalance. Additionally, it aids in studying genetic polymorphisms affecting enzyme activity and their association with disease susceptibility. Structural studies using recombinant protein have also contributed to understanding its catalytic mechanism and designing targeted therapies. Overall, CYP26B1 recombinant protein serves as a crucial tool for unraveling RA-mediated pathways and developing precision medicine approaches.
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