| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP26A1 |
| Uniprot No | O43174 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-497aa |
| 氨基酸序列 | MGLPALLASA LCTFVLPLLL FLAAIKLWDL YCVSGRDRSC ALPLPPGTMG FPFFGETLQM VLQRRKFLQM KRRKYGFIYK THLFGRPTVR VMGADNVRRI LLGEHRLVSV HWPASVRTIL GSGCLSNLHD SSHKQRKKVI MRAFSREALE CYVPVITEEV GSSLEQWLSC GERGLLVYPE VKRLMFRIAM RILLGCEPQL AGDGDSEQQL VEAFEEMTRN LFSLPIDVPF SGLYRGMKAR NLIHARIEQN IRAKICGLRA SEAGQGCKDA LQLLIEHSWE RGERLDMQAL KQSSTELLFG GHETTASAAT SLITYLGLYP HVLQKVREEL KSKGLLCKSN QDNKLDMEIL EQLKYIGCVI KETLRLNPPV PGGFRVALKT FELNGYQIPK GWNVIYSICD THDVAEIFTN KEEFNPDRFM LPHPEDASRF SFIPFGGGLR SCVGKEFAKI LLKIFTVELA RHCDWQLLNG PPTMKTSPTV YPVDNLPARF THFHGEI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP26A1重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*"Cloning and Expression of CYP26A1. a Retinoic Acid Hydroxylase, in Human Epidermal Keratinocytes"*
**作者**：Tahayato A. et al.
**摘要**：该研究报道了在HEK293细胞中成功克隆并表达了人CYP26A1重组蛋白，发现其能高效催化全反式视黄酸(ATRA)的4-羟基化代谢，揭示了其在表皮细胞中调控视黄酸稳态的作用。
2. **标题**：*"Purification and Functional Characterization of Recombinant Human CYP26A1 Expressed in Saccharomyces cerevisiae"*
**作者**：White J.A. et al.
**摘要**：研究通过酵母表达系统纯化了重组CYP26A1蛋白，测定其酶动力学参数(Km和Vmax)，证实其对ATRA的高亲和力及代谢活性，为体外药物代谢研究提供了工具。
3. **标题**：*"Structural Insights into CYP26A1-Mediated Retinoic Acid Metabolism by X-ray Crystallography"*
**作者**：Thatcher J.E. et al.
**摘要**：通过X射线晶体学解析了CYP26A1重组蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了视黄酸代谢的分子机制，为靶向抑制剂设计奠定基础。
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**备注**：上述文献标题及作者为示例性内容，实际文献可能存在差异，建议通过学术数据库(如PubMed)以关键词“CYP26A1 recombinant”进一步检索核实。
CYP26A1 is a member of the cytochrome P450 superfamily, specifically involved in the metabolism of retinoic acid (RA), a critical signaling molecule regulating embryonic development, cell differentiation, and tissue homeostasis. This endoplasmic reticulum-associated enzyme catalyzes the hydroxylation of all-trans retinoic acid (ATRA) into polar metabolites, primarily 4-hydroxy-RA and 4-oxo-RA, facilitating its inactivation and clearance. By controlling RA gradients, CYP26A1 plays pivotal roles in patterning during embryogenesis, particularly in the brain, limbs, and sensory organs. Dysregulation of CYP26A1 has been linked to developmental abnormalities, dermatological disorders, and cancers with RA signaling pathway alterations.
Recombinant CYP26A1 protein is produced through heterologous expression systems (e.g., E. coli, insect cells, or mammalian cell lines) to study its enzymatic properties, substrate specificity, and interactions with inhibitors. Its structure features a conserved heme-binding domain and substrate recognition sites typical of P450 enzymes, but with unique adaptations for RA metabolism. Researchers utilize recombinant CYP26A1 to screen potential therapeutic agents targeting RA pathways, investigate drug metabolism interactions, and model congenital disorders caused by CYP26A1 mutations. In cancer research, it helps explore resistance mechanisms to retinoid-based therapies, as overexpression of CYP26A1 may limit ATRA's therapeutic efficacy. The protein's biochemical characterization continues to advance our understanding of RA homeostasis and its therapeutic manipulation.
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