| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX4I1 |
| Uniprot No | P13073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-169aa |
| 氨基酸序列 | AHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMWQKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK |
| 预测分子量 | 33.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COX4I1重组蛋白的3篇示例参考文献(注：内容为模拟概括，非真实文献)：
1. **文献名称**：*Recombinant COX4I1 Expression and Structural Insights into Mitochondrial Complex IV Assembly*
**作者**：Lee et al.
**摘要**：研究通过大肠杆菌系统表达并纯化人源COX4I1重组蛋白，结合X射线晶体学解析其三维结构，揭示其在细胞色素c氧化酶复合体IV组装中的关键作用。
2. **文献名称**：*COX4I1 Hypoxia-Induced Modulation: Role of Recombinant Protein in Cellular Energy Metabolism*
**作者**：Zhang et al.
**摘要**：利用哺乳动物细胞表达体系制备功能性COX4I1重组蛋白，证明其在低氧条件下通过稳定线粒体呼吸链活性调节细胞能量代谢，并影响ROS生成。
3. **文献名称**：*Functional Characterization of COX4I1 Mutants in Lung Cancer Using Recombinant Protein Models*
**作者**：Martinez et al.
**摘要**：通过重组COX4I1蛋白研究其肺癌相关突变体功能，发现特定突变导致酶活性下降，并与肿瘤细胞异常增殖和化疗耐药性相关。
(注：以上为基于学术领域常见主题的模拟内容，实际文献需通过数据库如PubMed检索确认。)
COX4I1 (Cytochrome c Oxidase Subunit 4 Isoform 1) is a nuclear-encoded structural component of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (Complex IV). This enzyme catalyzes the transfer of electrons from cytochrome c to molecular oxygen, a critical step in aerobic respiration that drives ATP synthesis. COX4I1 is one of two isoforms of subunit 4 (COX4I1 and COX4I2), with COX4I1 being ubiquitously expressed across tissues, while COX4I2 is tissue-specific and hypoxia-inducible.
The COX4I1 protein plays a regulatory role in COX assembly, stability, and enzymatic activity. It contains a conserved N-terminal domain that interacts with other COX subunits and facilitates the enzyme’s conformational changes during catalysis. Studies suggest COX4I1 expression is tightly linked to cellular energy demands and oxygen availability. Under hypoxic conditions, its downregulation or replacement by COX4I2 may optimize COX function for low-oxygen environments, influencing metabolic adaptation.
Recombinant COX4I1 protein is produced using expression systems like *E. coli* or mammalian cell cultures, often tagged for purification (e.g., His-tag). Its recombinant form enables biochemical studies, including COX activity assays, protein-protein interaction mapping, and structural analyses via X-ray crystallography or cryo-EM. Researchers also use it to explore disease mechanisms, as COX dysfunction is implicated in neurodegenerative disorders, cancer metabolism, and mitochondrial diseases. For example, elevated COX4I1 levels in certain cancers correlate with enhanced oxidative phosphorylation, supporting tumor proliferation.
Additionally, recombinant COX4I1 serves as a tool to investigate isoform-specific roles in hypoxia response pathways and ROS regulation. Its study may inform therapeutic strategies targeting mitochondrial energy metabolism or hypoxia-related pathologies.
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