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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLCF1 |
| Uniprot No | Q9UBD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-225aa |
| 氨基酸序列 | MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLCF1重组蛋白的3篇参考文献示例(注：以下文献为示例性内容，实际引用时请核实真实来源)：
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1. **标题**: *Production and Functional Analysis of Recombinant Human CLCF1 in a Prokaryotic System*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人CLCF1蛋白，通过体外实验证实其能够特异性结合CNTFR/IL-27Rα/gp130受体复合物，并激活STAT3信号通路，为CLCF1的神经保护功能研究提供了工具。
2. **标题**: *CLCF1 Recombinant Protein Attenuates Hepatic Fibrosis via Modulation of TGF-β Signaling*
**作者**: Zhang Y, et al.
**摘要**: 在小鼠肝纤维化模型中，重组CLCF1蛋白通过抑制TGF-β/Smad3通路显著减少胶原沉积，表明其潜在的抗纤维化治疗应用价值。
3. **标题**: *Structural Characterization and Receptor Binding Specificity of CLCF1*
**作者**: Lee H, et al.
**摘要**: 通过冷冻电镜技术解析了重组CLCF1蛋白与受体复合物的三维结构，揭示了其结合界面关键氨基酸残基，为靶向药物设计提供了结构基础。
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如需具体文献，建议在PubMed或Web of Science中以“CLCF1 recombinant protein”为关键词检索，并筛选近年发表的实验研究。
CLCF1 (Cardiotrophin-like Cytokine Factor 1) is a secreted protein belonging to the interleukin-6 (IL-6) cytokine family, characterized by its four-α-helical bundle structure. It was initially identified as a neurotrophic and immunomodulatory factor, encoded by the *CLCF1* gene located on human chromosome 11q13. CLCF1 functions by forming a heterodimeric complex with either cytokine receptor-like factor 1 (CRLF1) or soluble ciliary neurotrophic factor receptor (sCNTFR), which stabilizes its structure and enables binding to the ciliary neurotrophic factor receptor (CNTFR). This ternary complex subsequently interacts with glycoprotein 130 (gp130) and leukemia inhibitory factor receptor β (LIFRβ), activating downstream signaling pathways such as JAK/STAT, MAPK, and PI3K/AKT. These pathways regulate cellular processes including neuronal survival, metabolism, and immune responses.
Physiologically, CLCF1 is implicated in neuronal development, metabolic homeostasis, and immune regulation. It supports the survival of sensory and motor neurons and modulates synaptic plasticity. In metabolic contexts, CLCF1 influences adipocyte differentiation and insulin sensitivity. Dysregulation of CLCF1 has been linked to pathologies such as autoimmune disorders (e.g., rheumatoid arthritis), inflammatory conditions, and certain cancers. Elevated CLCF1 levels correlate with chronic inflammation due to its role in promoting pro-inflammatory cytokine secretion. Conversely, reduced expression is observed in neurodegenerative diseases, suggesting a protective role in neuronal maintenance.
Recombinant CLCF1 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Purification involves affinity and ion-exchange chromatography. As a research tool, it aids in studying cytokine signaling mechanisms, drug screening for inflammatory/neurological diseases, and exploring therapeutic applications in tissue repair or immune modulation. Its dual role in inflammation and neuroprotection makes it a molecule of interest for targeted therapies.
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