| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX3 |
| Uniprot No | P23219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-599aa |
| 氨基酸序列 | ADPGAPT PVNPCCYYPC QHQGICVRFG LDRYQCDCTR TGYSGPNCTI PGLWTWLRNS LRPSPSFTHF LLTHGRWFWE FVNATFIREM LMRLVLTVRS NLIPSPPTYN SAHDYISWES FSNVSYYTRI LPSVPKDCPT PMGTKGKKQL PDAQLLARRF LLRRKFIPDP QGTNLMFAFF AQHFTHQFFK TSGKMGPGFT KALGHGVDLG HIYGDNLERQ YQLRLFKDGK LKYQVLDGEM YPPSVEEAPV LMHYPRGIPP QSQMAVGQEV FGLLPGLMLY ATLWLREHNR VCDLLKAEHP TWGDEQLFQT TRLILIGETI KIVIEEYVQQ LSGYFLQLKF DPELLFGVQF QYRNRIAMEF NHLYHWHPLM PDSFKVGSQE YSYEQFLFNT SMLVDYGVEA LVDAFSRQIA GRIGGGRNMD HHILHVAVDV IRESREMRLQ PFNEYRKRFG MKPYTSFQEL VGEKEMAAEL EELYGDIDAL EFYPGLLLEK CHPNSIFGES MIEIGAPFSL KGLLGNPICS PEYWKPSTFG GEVGFNIVKT ATLKKLVCLN TKTCPYVSFR VPDASQDDGP AVERPSTEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COX3重组蛋白的3篇参考文献示例(注：内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*Heterologous Expression and Functional Characterization of Mitochondrial Cytochrome c Oxidase Subunit III (COX3) in Saccharomyces cerevisiae*
**作者**：Lee S., et al.
**摘要**：研究通过酵母表达系统成功重组表达了COX3蛋白，并验证其与线粒体复合物IV其他亚基的组装能力，证明重组COX3可部分恢复呼吸缺陷型酵母的氧化磷酸化功能。
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2. **文献名称**：*Purification and Structural Analysis of Recombinant COX3 for Insights into Proton Channel Dynamics*
**作者**：Zhang Y., et al.
**摘要**：利用大肠杆菌表达系统纯化COX3重组蛋白，结合冷冻电镜技术解析其三维结构，揭示了COX3在质子传递中的关键氨基酸残基及潜在调控机制。
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3. **文献名称**：*COX3 Overexpression via Recombinant Technology Attenuates Ischemia-Reperfusion Injury in Cardiomyocytes*
**作者**：Wang H., et al.
**摘要**：通过哺乳动物细胞表达重组COX3蛋白，发现其可减少心肌细胞氧化应激损伤，机制可能与维持线粒体膜电位及抑制凋亡通路相关。
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**提示**：以上信息为基于领域知识的模拟生成，实际文献建议通过PubMed、Google Scholar等平台以“COX3 recombinant protein”或“Cytochrome c oxidase subunit 3 expression”为关键词检索，并重点关注近5年的结构生物学、线粒体疾病相关研究。
Cytochrome c oxidase subunit III (COX3) is a critical component of Complex IV in the mitochondrial electron transport chain (ETC), which drives cellular ATP production through oxidative phosphorylation. Encoded by mitochondrial DNA (mtDNA), COX3 anchors the catalytic core of cytochrome c oxidase, facilitating the transfer of electrons from cytochrome c to molecular oxygen, coupled with proton pumping across the inner mitochondrial membrane. This process is essential for maintaining the proton gradient required for ATP synthesis.
Recombinant COX3 protein is engineered to study its structural and functional roles, particularly in contexts where mitochondrial dysfunction contributes to diseases such as neurodegenerative disorders, metabolic syndromes, and cancer. Producing COX3 recombinantly involves cloning its gene into expression systems (e.g., bacterial, yeast, or mammalian cells) to overcome challenges in isolating the native protein from mitochondria. However, its hydrophobic nature and membrane-associated properties often necessitate fusion tags or specialized vectors for solubility and proper folding.
Research using recombinant COX3 focuses on elucidating mechanisms of ETC regulation, apoptosis, and reactive oxygen species (ROS) modulation. It also aids in modeling mtDNA mutations linked to pathologies like Leber’s hereditary optic neuropathy (LHON) or Leigh syndrome. Additionally, COX3 serves as a tool for drug screening targeting mitochondrial disorders or metabolic reprogramming in cancer. Advances in protein engineering and structural biology (e.g., cryo-EM) have enhanced the study of COX3’s interactions within Complex IV, offering insights into therapeutic strategies for mitochondrial-related diseases.
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