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Recombinant Human ICAM3 protein

  • 中文名: 细胞间粘附分子3(ICAM3)重组蛋白
  • 别    名: ICAM3;Intercellular adhesion molecule 3
货号: PA1000-9015
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ICAM3
Uniprot NoP32942
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-485aa
氨基酸序列MATMVPSVLW PRACWTLLVC CLLTPGVQGQ EFLLRVEPQN PVLSAGGSLF VNCSTDCPSS EKIALETSLS KELVASGMGW AAFNLSNVTG NSRILCSVYC NGSQITGSSN ITVYRLPERV ELAPLPPWQP VGQNFTLRCQ VEDGSPRTSL TVVLLRWEEE LSRQPAVEEP AEVTATVLAS RDDHGAPFSC RTELDMQPQG LGLFVNTSAP RQLRTFVLPV TPPRLVAPRF LEVETSWPVD CTLDGLFPAS EAQVYLALGD QMLNATVMNH GDTLTATATA TARADQEGAR EIVCNVTLGG ERREARENLT VFSFLGPIVN LSEPTAHEGS TVTVSCMAGA RVQVTLDGVP AAAPGQPAQL QLNATESDDG RSFFCSATLE VDGEFLHRNS SVQLRVLYGP KIDRATCPQH LKWKDKTRHV LQCQARGNPY PELRCLKEGS SREVPVGIPF FVNVTHNGTY QCQASSSRGK YTLVVVMDIE AGSSH
预测分子量77 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ICAM3重组蛋白的3篇参考文献,按文献名称、作者和摘要内容简要概括:

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1. **文献名称**:*Expression and functional characterization of recombinant human intercellular adhesion molecule-3 (ICAM-3) in eukaryotic cells*

**作者**:Fawcett J, et al.

**摘要**:该研究报道了在哺乳动物细胞中成功表达重组人ICAM3蛋白,并验证其与淋巴细胞表面配体LFA-1的结合活性,证明其在免疫细胞黏附和信号转导中的关键作用。

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2. **文献名称**:*ICAM-3 mediates leukocyte adhesion to endothelial cells through a mechanism distinct from ICAM-1 and ICAM-2*

**作者**:de Fougerolles AR, et al.

**摘要**:作者通过重组ICAM3蛋白实验,发现其通过不同于ICAM1/2的路径介导白细胞与内皮细胞的黏附,提示其在炎症反应中的独特调控功能。

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3. **文献名称**:*Structural analysis of ICAM-3 reveals its role in HIV-1 infection via interaction with dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN)*

**作者**:van Gils MJ, et al.

**摘要**:研究利用重组ICAM3蛋白解析其与DC-SIGN的结合结构,阐明其在HIV-1病毒通过树突状细胞传播中的分子机制,为抗病毒治疗提供新靶点。

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4. **文献名称**:*Recombinant ICAM-3 modulates neutrophil activation and apoptosis via CD11b/CD18 integrin interaction*

**作者**:Mócsai A, et al.

**摘要**:通过重组ICAM3蛋白实验,揭示其与中性粒细胞表面CD11b/CD18整合素结合后调控细胞活化与凋亡的分子通路,为炎症性疾病研究提供新方向。

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以上文献均聚焦于重组ICAM3蛋白的表达、功能及结构机制,涵盖免疫调控、病原感染和细胞信号传导等领域。

背景信息

ICAM-3 (Intercellular Adhesion Molecule 3), also known as CD50. is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It is constitutively expressed on leukocytes, particularly T cells, neutrophils, and antigen-presenting cells, playing a critical role in immune regulation and cell-cell interactions. Structurally, ICAM-3 consists of five extracellular Ig-like domains, a transmembrane region, and a short cytoplasmic tail. It serves as a ligand for lymphocyte function-associated antigen 1 (LFA-1. CD11a/CD18) and dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), facilitating leukocyte adhesion, immune synapse formation, and T-cell activation.

Recombinant ICAM-3 proteins are engineered versions of the native protein, typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation. These proteins often include the extracellular domain (amino acids 1-460) to retain binding functionality while excluding the transmembrane and cytoplasmic regions for solubility. Purification methods commonly involve affinity tags like Fc-fusion or His-tags.

The development of recombinant ICAM-3 has enabled detailed studies of its immunological roles, including its involvement in HIV-1 infection (via DC-SIGN-mediated viral capture) and autoimmune diseases. It is also used to screen therapeutic agents targeting inflammatory pathways or to develop diagnostic tools for immune disorders. Additionally, ICAM-3 recombinant proteins serve as critical reagents in flow cytometry, ELISA, and cell adhesion assays to dissect leukocyte interactions. Their stability and standardized activity make them valuable for both basic research and translational applications in immunology and immunotherapy.

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