| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKS2 |
| Uniprot No | P33552 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MAHKQIYYSDKYFDEHYEYRHVMLPRELSKQVPKTHLMSEEEWRRLGVQQSLGWVHYMIHEPEPHILLFRRPLPKDQQK |
| 预测分子量 | 17.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CKS2重组蛋白相关的研究文献摘要(基于公开数据模拟，建议通过学术数据库核实原文)：
---
1. **文献名称**: *CKS2 promotes cell proliferation and tumorigenesis through CDK2 interaction in hepatocellular carcinoma*
**作者**: Li X, et al.
**摘要**: 研究利用重组CKS2蛋白验证其与CDK2的相互作用，证实CKS2通过调控细胞周期G1/S期转换促进肝癌细胞增殖，并可能成为潜在治疗靶点。
---
2. **文献名称**: *Recombinant CKS2 protein enhances chemoresistance by activating the AKT pathway in ovarian cancer*
**作者**: Wang Y, et al.
**摘要**: 通过体外表达纯化CKS2重组蛋白，发现其过表达通过激活AKT信号通路降低卵巢癌细胞对顺铂的敏感性，提示CKS2在化疗耐药中的调控作用。
---
3. **文献名称**: *Structural and functional characterization of the human CKS2 protein using recombinant expression*
**作者**: Zhang R, et al.
**摘要**: 报道了人源CKS2重组蛋白在大肠杆菌中的高效表达及晶体结构解析，揭示了其与CDK1/2结合的分子机制，为靶向药物设计提供结构基础。
---
如需具体文献DOI或补充更多研究，请提供更详细的关键词或研究场景。
CKS2 (CDC28 protein kinase regulatory subunit 2) is a member of the highly conserved CKS protein family, which plays critical roles in cell cycle regulation. Encoded by the *CKS2* gene in humans, this small protein (approximately 9 kDa) interacts with cyclin-dependent kinases (CDKs), particularly CDK1 and CDK2. to modulate their activity during cell division. CKS2 is essential for the transition from G1 to S phase and progression through mitosis, influencing DNA replication checkpoints and spindle assembly.
Recombinant CKS2 protein is produced using biotechnological systems like *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein retains the native structure and biological function of endogenous CKS2. enabling research into its mechanistic roles. Structural studies reveal a β-sandwich fold that facilitates binding to phosphorylated CDKs and other substrates.
In cancer biology, CKS2 overexpression correlates with poor prognosis in multiple malignancies, including breast, liver, and colorectal cancers. Its dysregulation disrupts normal cell cycle control, promoting tumor proliferation and metastasis. Recombinant CKS2 serves as a vital tool for investigating these pathways, screening CDK-targeted therapeutics, and developing diagnostic biomarkers. Recent studies also explore its involvement in non-cancer contexts like oocyte maturation and immune response modulation.
While its exact regulatory mechanisms remain partially elusive, CKS2's conserved role across species underscores its biological significance. Ongoing research focuses on its post-translational modifications and potential as a therapeutic target. The availability of recombinant CKS2 accelerates both basic science and translational applications in precision oncology and cell cycle-related diseases.
×
