| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT9B |
| Uniprot No | O14905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MRPPPALALAGLCLLALPAAAASYFGLTGREVLTPFPGLGTAAAPAQGGA HLKQCDLLKLSRRQKQLCRREPGLAETLRDAAHLGLLECQFQFRHERWNC SLEGRTGLLKRGFKETAFLYAVSSAALTHTLARACSAGRMERCTCDDSPG LESRQAWQWGVCGDNLKYSTKFLSNFLGSKRGNKDLRARADAHNTHVGIK AVKSGLRTTCKCHGVSGSCAVRTCWKQLSPFRETGQVLKLRYDSAVKVSS ATNEALGRLELWAPARQGSLTKGLAPRSGDLVYMEDSPSFCRPSKYSPGT AGWSAVARSQLITTSTSRIQAILPSQLPE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT9B重组蛋白的3篇文献示例(注：以下内容为模拟概括，实际文献需通过学术数据库核实)：
1. **文献名称**：*WNT9B signaling regulates kidney development through receptor-mediated β-catenin activation*
**作者**：Carroll, T.J., et al.
**摘要**：该研究利用重组WNT9B蛋白处理体外培养的肾脏细胞，证实其通过激活经典WNT/β-catenin信号通路，促进肾小管形态发生和细胞极性形成，揭示了WNT9B在胚胎肾脏发育中的关键作用。
2. **文献名称**：*Recombinant WNT9B enhances osteogenic differentiation of mesenchymal stem cells in vitro*
**作者**：Li, Y., et al.
**摘要**：作者通过大肠杆菌表达系统制备了高纯度重组WNT9B蛋白，并证明其在体外能显著促进间充质干细胞的成骨分化，为骨组织再生治疗提供了潜在分子工具。
3. **文献名称**：*Production and functional characterization of human WNT9B recombinant protein in mammalian cells*
**作者**：Garriock, R.J., et al.
**摘要**：该文献详细描述了在HEK293细胞中重组表达人源WNT9B蛋白的优化流程，包括糖基化修饰和生物活性验证，证实其能特异性结合FZD受体并激活下游信号。
4. **文献名称**：*WNT9B suppresses gastric cancer progression by inhibiting epithelial-mesenchymal transition*
**作者**：Wang, H., et al.
**摘要**：研究使用重组WNT9B蛋白处理胃癌细胞，发现其通过抑制WNT/β-catenin非经典通路，降低EMT标志物表达，从而减缓肿瘤转移，提示其潜在抗癌应用价值。
建议通过PubMed或Google Scholar以关键词“WNT9B recombinant”检索最新文献获取准确信息。
WNT9B is a member of the Wnt protein family, a group of evolutionarily conserved signaling molecules critical for embryonic development, tissue homeostasis, and cell fate regulation. As a secreted glycoprotein, WNT9B typically binds to cell surface receptors like Frizzled and LRP5/6. activating canonical Wnt/β-catenin or non-canonical signaling pathways. It plays essential roles in organogenesis, particularly in kidney, bone, and reproductive tract development. Studies highlight its involvement in ureteric bud branching during kidney formation and limb patterning.
Recombinant WNT9B protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper post-translational modifications, such as lipidylation, which is vital for its biological activity. Purification often involves affinity tags (e.g., His-tag) and chromatography. The recombinant form enables controlled in vitro studies, bypassing the complexity of endogenous Wnt secretion.
Researchers utilize WNT9B recombinant protein to investigate developmental mechanisms, tissue regeneration, and disease pathways, including congenital anomalies or cancers linked to Wnt dysregulation. Its application extends to organoid culture systems, where Wnt signaling directs stem cell differentiation. Challenges remain in maintaining protein stability and solubility due to its hydrophobic nature. Ongoing research aims to optimize delivery methods and explore therapeutic potentials in regenerative medicine.
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