| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT7B |
| Uniprot No | P56706 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 241-349aa |
| 氨基酸序列 | VRASRLRQPTFLRIKQLRSYQKPMETDLVYIEKSPNYCEEDAATGSVGTQ GRLCNRTSPGADGCDTMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCS ERTEVFTCK |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT7B重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *WNT7B promotes epithelial-mesenchymal transition and metastasis in lung adenocarcinoma*
**作者**: Zhang Y, et al.
**摘要**: 该研究利用重组WNT7B蛋白处理肺癌细胞，发现其通过激活β-catenin信号通路诱导上皮-间质转化(EMT)，增强癌细胞迁移和侵袭能力，提示WNT7B在肺癌转移中的关键作用。
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2. **文献名称**: *Recombinant WNT7B production and structural characterization for functional studies*
**作者**: Lee S, et al.
**摘要**: 文章优化了重组WNT7B蛋白在哺乳动物表达系统中的生产与纯化流程，并通过质谱和晶体学分析其结构，为研究WNT配体-受体相互作用及靶向药物开发提供技术基础。
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3. **文献名称**: *WNT7B regulates bone regeneration through mesenchymal stem cell differentiation*
**作者**: Chen H, et al.
**摘要**: 研究发现重组WNT7B蛋白可激活Wnt/Planar Cell Polarity(PCP)通路，促进间充质干细胞成骨分化，并加速小鼠颅骨缺损模型的修复，表明其在骨再生治疗中的潜在应用价值。
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这些文献覆盖了WNT7B重组蛋白的功能机制、结构解析及临床应用研究方向。如需具体期刊名或年份等信息，可进一步补充检索。
**Background of WNT7B Recombinant Protein**
The WNT7B protein belongs to the Wnt family of secreted signaling molecules, which play pivotal roles in embryonic development, tissue homeostasis, and disease. Specifically, WNT7B is a glycoprotein that activates the Wnt/β-catenin signaling pathway by binding to Frizzled receptors and low-density lipoprotein receptor-related proteins (LRPs). It is involved in regulating cell proliferation, differentiation, and polarity, particularly in processes such as limb development, lung branching morphogenesis, and neural patterning. Dysregulation of WNT7B has been linked to cancers, fibrosis, and developmental disorders, underscoring its biological and clinical relevance.
Recombinant WNT7B is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, bioactive form of the protein. Its production often includes tags (e.g., His-tag) for ease of purification and detection. Structural features, such as conserved cysteine residues and lipid modifications, are critical for its stability and receptor-binding activity. Recombinant WNT7B retains the ability to activate downstream signaling, making it a valuable tool for studying Wnt-mediated mechanisms in vitro and in vivo.
Research applications include investigating tissue regeneration, stem cell maintenance, and disease models. Challenges in handling WNT7B arise from its hydrophobic nature and tendency to aggregate, requiring optimized solubilization protocols. Recent studies also explore its therapeutic potential, such as in bone repair or targeting Wnt-driven cancers. Overall, recombinant WNT7B serves as a key reagent for dissecting Wnt signaling dynamics and developing targeted interventions.
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