| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT2B |
| Uniprot No | Q93097 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MLRPGGAEEAAQLPLRRASAPVPVPSPAAPDGSRASARLGLACLLLLLLL TLPARVDTSWWYIGALGARVICDNIPGLVSRQRQLCQRYPDIMRSVGEGA REWIRECQHQFRHHRWNCTTLDRDHTVFGRVMLRSSREAAFVYAISSAGV VHAITRACSQGELSVCSCDPYTRGRHHDQRGDFDWGGCSDNIHYGVRFAK AFVDAKEKRLKDARALMNLHNNRCGRTAVRRFLKLECKCHGVSGSCTLRT CWRALSDFRRTGDYLRRRYDGAVQVMATQDGANFTAARQGYRRATRTDLV YFDNSPDYCVLDKAAGSLGTAGRVCSKTSKGTDGCEIMCCGRGYDTTRVT RVTQCECKFHWCCAVRCKECRNTVDVHTCKAPKKAEWLDQT |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT2B重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *WNT2B regulates epithelial progenitor cell growth and differentiation in murine lung development*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组WNT2B蛋白处理小鼠肺上皮祖细胞，发现其激活经典Wnt/β-catenin信号通路，促进细胞增殖并抑制过早分化，表明WNT2B在肺形态发生中调控干细胞动态平衡的关键作用。
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2. **文献名称**: *Recombinant WNT2B Secretion and Signaling in Colorectal Cancer Models*
**作者**: Tanaka T, et al.
**摘要**: 作者利用HEK293细胞表达重组WNT2B蛋白，证明其通过旁分泌机制增强结直肠癌细胞侵袭性，并诱导EMT表型，提示WNT2B过表达可能作为癌症转移的生物标志物。
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3. **文献名称**: *Purification and functional characterization of human WNT2B for stem cell applications*
**作者**: Smith JR, et al.
**摘要**: 该研究优化了人源WNT2B重组蛋白在哺乳动物悬浮细胞中的表达工艺，证实其维持胚胎干细胞多能性的活性，为再生医学中Wnt蛋白制剂的标准化提供技术参考。
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备注：以上文献为示例性质，实际引用时请核对真实出版物信息。若需具体文献，建议在PubMed或Web of Science中检索关键词“WNT2B recombinant protein”。
**Background of WNT2B Recombinant Protein**
The WNT2B protein is a member of the Wnt family of secreted glycoproteins, which play critical roles in embryonic development, tissue homeostasis, and cellular processes such as proliferation, differentiation, and migration. Wnt proteins signal through both canonical (β-catenin-dependent) and non-canonical pathways, with WNT2B primarily associated with the canonical pathway. It binds to Frizzled receptors and low-density lipoprotein receptor-related proteins (LRP5/6), activating downstream signaling cascades that regulate gene expression. Dysregulation of Wnt signaling, including WNT2B, is implicated in developmental disorders, cancer, and other diseases.
Recombinant WNT2B is engineered *in vitro* using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as lipidylation, which is essential for its biological activity. This purified protein retains the ability to activate Wnt signaling pathways in target cells, making it a valuable tool for studying mechanisms in stem cell biology, tissue regeneration, and disease modeling.
Research applications include investigating WNT2B's role in maintaining stem cell pluripotency, promoting epithelial-mesenchymal transition (EMT) in cancer, and regulating organogenesis. In cancer, WNT2B overexpression is linked to tumor progression, metastasis, and therapy resistance, highlighting its potential as a therapeutic target. Additionally, recombinant WNT2B is used in *in vitro* assays to screen for Wnt pathway modulators or to optimize differentiation protocols for regenerative medicine.
Quality control of the recombinant protein involves verifying bioactivity through cell-based reporter assays (e.g., TOPFlash for β-catenin activation) and detecting phosphorylation of downstream targets like GSK3β. Its standardized production ensures reproducibility in experimental settings, bridging basic research and translational applications.
In summary, WNT2B recombinant protein serves as a critical reagent for dissecting Wnt signaling dynamics and developing targeted interventions in development and disease.
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