| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT2 |
| Uniprot No | P09544 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MNAPLGGIWLWLPLLLTWLTPEVNSSWWYMRATGGSSRVMCDNVPGLVSS QRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRV LLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKG IFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKR FLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDG TGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRG MDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKA PKNADWTTAT |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT2重组蛋白的3篇参考文献概览(示例为模拟内容，实际文献需根据具体研究补充)：
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1. **文献名称**: *WNT2 overexpression promotes tumor progression in colorectal cancer*
**作者**: Katoh M, et al.
**摘要**: 研究报道WNT2在结直肠癌中高表达，通过激活β-catenin信号通路促进肿瘤细胞增殖和侵袭。重组WNT2蛋白的体外实验证实其增强癌细胞迁移能力。
2. **文献名称**: *Recombinant WNT2 protein enhances self-renewal of hematopoietic stem cells*
**作者**: Willert K, et al.
**摘要**: 研究通过哺乳动物细胞表达系统制备重组WNT2蛋白，证明其能激活经典WNT通路，维持造血干细胞的长期自我更新能力，为干细胞培养提供关键因子。
3. **文献名称**: *WNT2-mediated regulation of pulmonary fibrosis through epithelial-mesenchymal transition*
**作者**: Liang J, et al.
**摘要**: 利用重组WNT2蛋白处理肺上皮细胞，发现其通过非经典WNT/Ca²⁺通路诱导上皮-间质转化(EMT)，提示WNT2在肺纤维化病理过程中的潜在作用。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“WNT2 recombinant protein”获取最新研究。
**Background of WNT2 Recombinant Protein**
The WNT family comprises evolutionarily conserved, secreted glycoproteins that regulate critical signaling pathways involved in embryonic development, tissue homeostasis, and cellular processes. WNT2. a member of this family, plays a role in modulating the Wnt/β-catenin signaling cascade, as well as β-catenin-independent pathways (e.g., planar cell polarity). It is implicated in organogenesis, particularly in lung and placental development, and has been linked to tissue repair, angiogenesis, and stem cell maintenance. Dysregulation of WNT2 is associated with diseases, including cancers, fibrosis, and developmental disorders, where it may act as an oncogene or tumor suppressor depending on context.
Recombinant WNT2 protein is engineered in vitro using expression systems (e.g., mammalian HEK293 cells) to ensure proper post-translational modifications, such as lipidylation, essential for its biological activity. This purified, bioactive form allows researchers to study WNT2-specific mechanisms without cross-reactivity from other Wnt ligands. Applications include *in vitro* cell signaling studies, organoid culture, and exploring therapeutic strategies targeting Wnt-driven pathologies. Challenges in handling WNT2 include its hydrophobicity and instability, often addressed using carrier proteins or specialized buffers.
As a research tool, recombinant WNT2 aids in deciphering its dual roles in health and disease, offering potential for regenerative medicine and anticancer therapies. However, its pleiotropic effects and complex signaling networks necessitate cautious interpretation of experimental outcomes.
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