| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT11 |
| Uniprot No | O96014 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-354aa |
| 氨基酸序列 | IKWLAL SKTPSALALN QTQHCKQLEG LVSAQVQLCR SNLELMHTVV HAAREVMKAC RRAFADMRWN CSSIELAPNY LLDLERGTRE SAFVYALSAA AISHAIARAC TSGDLPGCSC GPVPGEPPGP GNRWGGCADN LSYGLLMGAK FSDAPMKVKK TGSQANKLMR LHNSEVGRQA LRASLEMKCK CHGVSGSCSI RTCWKGLQEL QDVAADLKTR YLSATKVVHR PMGTRKHLVP KDLDIRPVKD SELVYLQSSP DFCMKNEKVG SHGTQDRQCN KTSNGSDSCD LMCCGRGYNP YTDRVVERCH CKYHWCCYVT CRRCERTVER YVCK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT11重组蛋白的3篇代表性文献摘要(虚构示例，仅供参考格式)：
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1. **文献名称**: "Efficient prokaryotic expression and purification of human WNT11 for functional studies"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了在大肠杆菌系统中重组表达人源WNT11蛋白的优化方法，通过融合标签提高可溶性，并验证其激活非经典Wnt/Ca²⁺信号通路的能力。
2. **文献名称**: "Recombinant WNT11 enhances cardiac progenitor cell migration in tissue regeneration"
**作者**: Smith JR, et al.
**摘要**: 利用哺乳动物细胞表达系统生产功能性WNT11重组蛋白，证明其通过调控细胞骨架重组促进心脏祖细胞迁移，为心脏修复提供潜在策略。
3. **文献名称**: "Structural analysis of WNT11-Frizzled interactions using purified recombinant proteins"
**作者**: Tanaka K, et al.
**摘要**: 通过体外重组WNT11与Frizzled受体蛋白结合实验，结合冷冻电镜技术解析互作界面，揭示WNT11特异性激活下游信号的分子机制。
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如需真实文献，建议检索PubMed或Google Scholar并筛选关键词“recombinant WNT11”。
**Background of WNT11 Recombinant Protein**
WNT11. a member of the Wnt family of secreted glycoproteins, plays critical roles in regulating cell signaling pathways during embryonic development, tissue homeostasis, and disease processes. It is primarily associated with the non-canonical Wnt/planar cell polarity (PCP) pathway, which coordinates cell polarity, directional migration, and tissue morphogenesis. WNT11 is also implicated in the Wnt/Ca²⁺ pathway, influencing cell adhesion, motility, and transcriptional regulation. Dysregulation of WNT11 has been linked to developmental anomalies, cancer progression, and cardiovascular disorders.
Recombinant WNT11 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, bioactive form of the protein. This allows researchers to study its structure, signaling mechanisms, and interactions with receptors like Frizzled (FZD) or co-receptors (e.g., ROR, RYK). The recombinant protein typically includes tags (e.g., His-tag) for efficient purification and detection.
Applications of recombinant WNT11 span basic research and therapeutic development. It is used to investigate its role in organogenesis (e.g., heart, kidney), neural crest cell migration, and cancer metastasis. In regenerative medicine, WNT11 has shown potential in enhancing stem cell differentiation and tissue repair. However, challenges remain in maintaining its stability and activity due to post-translational modifications (e.g., palmitoylation) critical for functionality.
Commercial recombinant WNT11 is often validated via bioassays (e.g., cell migration assays) and quality controls (e.g., SDS-PAGE, Western blot). Its study continues to shed light on Wnt signaling complexity and its therapeutic targeting in diseases like fibrosis, cardiomyopathy, and malignancies.
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