| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT10B |
| Uniprot No | O00744 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-389aa |
| 氨基酸序列 | NEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRNPDVTASALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQDRLRAKLLQLQALSRGKSFPHSLPSPGPGSSPSPGPQDTWEWGGCNHDMDFGEKFSRDFLDSREAPRDIQARMRIHNNRVGRQVVTENLKRKCKCHGTSGSCQFKTCWRAAPEFRAVGAALRERLGRAIFIDTHNRNSGAFQPRLRPRRLSGELVYFEKSPDFCERDPTMGSPGTRGRACNKTSRLLDGCGSLCCGRGHNVLRQTRVERCHCRFHWCCYVLCDECKVTEWVNVCK |
| 预测分子量 | 47.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNT10B重组蛋白的3篇示例文献摘要(内容为示例，建议通过PubMed等平台查询真实文献)：
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1. **文献名称**: *WNT10B enhances osteoblastogenesis through regulation of β-catenin signaling*
**作者**: Bennett CN, et al.
**摘要**: 研究利用重组WNT10B蛋白处理间充质干细胞，发现其通过激活经典Wnt/β-catenin信号通路显著促进成骨分化，并抑制脂肪生成，提示其在骨再生治疗中的潜在应用。
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2. **文献名称**: *Recombinant WNT10B protein maintains self-renewal of hematopoietic stem cells in vitro*
**作者**: Kawakami Y, et al.
**摘要**: 报道重组WNT10B蛋白通过结合Frizzled受体，激活非经典Wnt信号，有效维持造血干细胞的体外扩增能力，为干细胞治疗提供了新策略。
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3. **文献名称**: *WNT10B regulates adipogenesis via antagonizing PPARγ activity*
**作者**: Christodoulides C, et al.
**摘要**: 研究发现重组WNT10B蛋白可通过抑制PPARγ转录活性，调控前脂肪细胞分化，揭示其在代谢性疾病(如肥胖)中的分子机制。
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如需具体文献，建议在PubMed、Google Scholar等平台检索关键词“recombinant WNT10B protein”或结合研究领域(如骨代谢、癌症)筛选近期论文。
**Background of WNT10B Recombinant Protein**
WNT10B, a member of the Wnt family of secreted glycoproteins, plays critical roles in embryonic development, tissue homeostasis, and cellular processes such as proliferation, differentiation, and stem cell maintenance. It signals through the canonical β-catenin-dependent pathway, where it binds to Frizzled receptors and LRP co-receptors, stabilizing β-catenin to regulate target gene expression. WNT10B is particularly notable for its involvement in adipogenesis, skeletal development, and hair follicle formation. Dysregulation of WNT10B is linked to pathologies including osteoporosis, metabolic disorders, and cancers, highlighting its therapeutic and diagnostic potential.
Recombinant WNT10B protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce bioactive forms for research and therapeutic applications. The protein typically retains post-translational modifications (e.g., palmitoylation) essential for receptor interaction and solubility. Purification via affinity chromatography ensures high specificity, though challenges like protein stability and solubility persist.
In research, recombinant WNT10B is used to study Wnt signaling mechanisms, stem cell differentiation, and tissue regeneration. It has shown promise in enhancing osteogenic differentiation in bone repair models and suppressing adipogenesis in obesity-related studies. Additionally, it serves as a tool for developing targeted therapies, such as Wnt pathway inhibitors for cancer or activators for regenerative medicine. Despite its potential, complexities in Wnt signaling crosstalk and delivery hurdles in vivo require further optimization. Overall, recombinant WNT10B remains a vital reagent for dissecting Wnt biology and advancing translational applications.
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