| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT10A |
| Uniprot No | Q9GZT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-417aa |
| 氨基酸序列 | MPRSAPNDIL DLRLPPEPVL NANTVCLTLP GLSRRQMEVC VRHPDVAASA IQGIQIAIHE CQHQFRDQRW NCSSLETRNK IPYESPIFSR GFRESAFAYA IAAAGVVHAV SNACALGKLK ACGCDASRRG DEEAFRRKLH RLQLDALQRG KGLSHGVPEH PALPTASPGL QDSWEWGGCS PDMGFGERFS KDFLDSREPH RDIHARMRLH NNRVGRQAVM ENMRRKCKCH GTSGSCQLKT CWQVTPEFRT VGALLRSRFH RATLIRPHNR NGGQLEPGPA GAPSPAPGAP GPRRRASPAD LVYFEKSPDF CEREPRLDSA GTVGRLCNKS SAGSDGCGSM CCGRGHNILR QTRSERCHCR FHWCCFVVCE ECRITEWVSV CK |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与WNT10A重组蛋白相关的模拟参考文献(基于研究领域常见主题，非真实文献)：
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1. **标题**: *Expression and functional analysis of recombinant human WNT10A in stem cell osteogenic differentiation*
**作者**: Smith J, et al.
**摘要**: 报道了在哺乳动物细胞中表达WNT10A重组蛋白的方法，证明其通过激活经典WNT/β-catenin通路增强间充质干细胞的成骨分化。
2. **标题**: *Purification of bioactive WNT10A from E. coli and its role in hair follicle regeneration*
**作者**: Tanaka K, et al.
**摘要**: 优化大肠杆菌表达系统生产可溶性WNT10A重组蛋白，并验证其在毛囊干细胞激活中的功能，为脱发治疗提供依据。
3. **标题**: *WNT10A recombinant protein rescues dental defects in a murine model of ectodermal dysplasia*
**作者**: Chen L, et al.
**摘要**: 利用昆虫细胞表达重组WNT10A，通过动物实验证明其可部分逆转Wnt10a基因缺失导致的牙齿发育异常。
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**注**：以上为示例性内容，实际文献需通过PubMed或Google Scholar等平台检索关键词“recombinant WNT10A protein”获取。
**Background of WNT10A Recombinant Protein**
WNT10A is a secreted glycoprotein belonging to the Wnt family, which governs critical signaling pathways involved in embryonic development, tissue homeostasis, and cell fate determination. As a key ligand in both β-catenin-dependent (canonical) and -independent (non-canonical) Wnt signaling cascades, WNT10A regulates processes such as cell proliferation, differentiation, and polarity. Its role is particularly prominent in ectodermal tissue development, including skin, hair, teeth, and nails.
Recombinant WNT10A protein is engineered in vitro using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and folding. This bioactive form mimics native WNT10A, enabling researchers to study its function, receptor interactions (e.g., Frizzled and LRP5/6), and downstream signaling mechanisms.
Mutations in the *WNT10A* gene are linked to human pathologies, including ectodermal dysplasias (e.g., odonto-onycho-dermal dysplasia), tooth agenesis, and palmoplantar keratoderma. Dysregulated WNT10A expression is also implicated in cancers, fibrosis, and metabolic disorders, highlighting its dual role as a tumor promoter or suppressor depending on context.
The recombinant protein serves as a tool for exploring Wnt pathway modulation, drug screening, and regenerative therapies. For instance, it aids in studying stem cell maintenance, tissue repair, and bone regeneration. Challenges in WNT10A research include its hydrophobic nature, low solubility, and complex signaling crosstalk, often requiring carrier proteins (e.g., albumin) for stabilization.
Overall, WNT10A recombinant protein is vital for deciphering developmental biology and disease mechanisms, offering potential therapeutic avenues for Wnt-related disorders.
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