| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT1 |
| Uniprot No | P04628 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-370aa |
| 氨基酸序列 | ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与WNT1重组蛋白相关的3篇参考文献的简要概括(文献信息为示例性描述，非真实引用)：
1. **文献名称**：*WNT1 Recombinant Protein Enhances Osteogenic Differentiation of Mesenchymal Stem Cells*
**作者**：Smith A et al.
**摘要**：研究通过体外实验证明，外源性添加WNT1重组蛋白可通过激活经典Wnt/β-catenin通路，显著促进间充质干细胞的成骨分化，为骨组织再生治疗提供潜在策略。
2. **文献名称**：*Purification and Functional Characterization of Human WNT1 Recombinant Protein Expressed in Mammalian Cells*
**作者**：Chen L et al.
**摘要**：报道了一种在HEK293细胞中高效表达并纯化具有生物活性的人源WNT1重组蛋白的方法，验证其在细胞迁移和肿瘤生长调控中的功能。
3. **文献名称**：*WNT1 Signaling in Neural Development: Insights from Recombinant Protein-Based Models*
**作者**：Yamamoto K et al.
**摘要**：利用WNT1重组蛋白构建体外模型，揭示其在神经嵴细胞分化和中枢神经系统发育中的关键作用，并探讨相关分子机制。
(注：以上文献为模拟内容，实际引用需查询具体数据库如PubMed或Google Scholar获取真实信息。)
Wnt1 recombinant protein is a purified, biologically active form of the Wnt1 signaling molecule, a key member of the evolutionarily conserved Wnt family. Wnt proteins are secreted glycoproteins that regulate critical processes in embryonic development, tissue homeostasis, and stem cell maintenance. Specifically, Wnt1 was first identified as a proto-oncogene activated by mouse mammary tumor virus integration. It plays essential roles in neural tube formation, midbrain development, and bone metabolism through the canonical Wnt/β-catenin signaling pathway. Dysregulation of Wnt1 is associated with cancers, skeletal disorders, and neurodegenerative diseases.
Recombinant Wnt1 is produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, particularly lipidylation, which is crucial for its hydrophobic nature and receptor binding. The protein typically contains an N-terminal signal peptide for secretion and a C-terminal tag (e.g., His-tag) for purification. Researchers utilize it to activate Wnt signaling in vitro, studying mechanisms of tissue regeneration, cancer progression, and stem cell differentiation. In therapeutic contexts, Wnt1 shows potential for bone fracture repair and neurological disorder treatments, though challenges remain in delivery due to poor solubility and short half-life. Current studies focus on optimizing stabilization methods and developing Wnt1-based biologics for clinical applications.
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