| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DISP1 |
| Uniprot No | Q96F81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DISP1重组蛋白的参考文献示例(注：以下文献信息为模拟内容，实际引用时请核实原文准确性)：
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1. **文献名称**: *"Recombinant Production and Functional Characterization of the DISP1 Ectodomain in Hedgehog Signaling"*
**作者**: Kawakami T, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了DISP1蛋白的胞外结构域重组蛋白，通过体外结合实验证实其与Hedgehog(Hh)配体的直接相互作用，揭示了DISP1在Hh分泌中的关键作用。
2. **文献名称**: *"Structural Insights into DISP1-Mediated Cholesterol Transport via Cryo-EM Analysis"*
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物表达系统制备了全长DISP1重组蛋白，结合冷冻电镜技术解析了其跨膜结构域的三维结构，阐明了DISP1在胆固醇依赖性Hh蛋白转运中的分子机制。
3. **文献名称**: *"High-Throughput Screening of DISP1 Inhibitors Using a Recombinant Protein-Based Assay"*
**作者**: Smith JA, et al.
**摘要**: 通过昆虫细胞(Sf9)系统表达功能性DISP1重组蛋白，建立基于细胞外活性的药物筛选平台，鉴定了多个潜在小分子抑制剂，为靶向Hh信号通路的抗癌治疗提供新策略。
4. **文献名称**: *"Functional Analysis of DISP1 Mutations in Human Disease Using Recombinant Mutant Proteins"*
**作者**: Rodríguez EM, et al.
**摘要**: 构建了多种DISP1病理突变体的重组蛋白，发现特定突变导致其与Hh配体结合能力显著降低，解释了发育异常相关疾病的分子病理机制。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science或专业数据库检索最新文献。如需具体文献指导，建议补充研究背景或关键词进一步筛选。
**Background of DISP1 Recombinant Protein**
DISP1 (Dispatched homolog 1) is a transmembrane protein crucial for the Sonic Hedgehog (Shh) signaling pathway, which plays a pivotal role in embryonic development, tissue patterning, and cell differentiation. Originally identified in *Drosophila*, the Dispatched family proteins are responsible for releasing lipid-modified morphogens, such as Shh, from producing cells. DISP1 specifically facilitates the secretion and long-range transport of cholesterol-anchored Shh by solubilizing it and enabling its distribution across extracellular spaces. This process is essential for establishing gradients that direct cellular responses during organogenesis, particularly in the nervous system, limbs, and face.
Structurally, DISP1 contains a conserved N-terminal domain implicated in cholesterol transfer, a sterol-sensing domain, and multiple transmembrane regions. Mutations or dysregulation of DISP1 are linked to congenital disorders like holoprosencephaly (a brain malformation) and cancers due to disrupted Shh signaling.
Recombinant DISP1 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains functional domains necessary for studying Shh trafficking mechanisms. Researchers utilize it to investigate DISP1’s interaction with Shh, PTCH1 (receptor), or SCUBE2 (a co-factor), as well as its role in diseases. Its application spans *in vitro* binding assays, cell-based signaling studies, and animal models to explore therapeutic strategies targeting Shh-driven pathologies.
The development of DISP1 recombinant protein has advanced drug discovery, enabling high-throughput screens for inhibitors or enhancers of Shh secretion. Its study also sheds light on conserved pathways, such as Wnt signaling, broadening its relevance in developmental biology and regenerative medicine.
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