| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKB |
| Uniprot No | P12277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSG FTLDDVIQTG VDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHG GYKPSDEHKTDLNPDNLQGGDD LDPNYVLSSRVRTGRSIRGFCLPPHC SRGERRAIEKLAVEALSSLDGDLAGRYYALKSMT EAEQQQLIDDHFLF DKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISM QK GGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLR AGVHIKLP NLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNAD RLGFSEVELVQMVVDGVKLL IEMEQRLEQGQAIDDLMPAQK |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKB(肌酸激酶脑型)重组蛋白的模拟参考文献示例(文献信息为假设，供参考)：
---
1. **文献名称**：*Expression and Purification of Recombinant Human Brain-Type Creatine Kinase in Escherichia coli*
**作者**：Zhang Y. et al.
**摘要**：研究报道了通过大肠杆菌表达系统高效生产重组人CKB蛋白的方法，利用His标签进行亲和层析纯化，并验证了重组蛋白的酶活性和稳定性，为CKB的功能研究提供工具。
2. **文献名称**：*Structural Insights into CKB: Crystallographic Analysis of Recombinant Protein*
**作者**：Smith J.R. et al.
**摘要**：通过X射线晶体学解析了重组CKB的三维结构，揭示了其活性位点及ATP/肌酸结合区域，为开发靶向CKB的抑制剂奠定结构基础。
3. **文献名称**：*Role of Recombinant CKB in Cancer Cell Energy Metabolism*
**作者**：Lee H. et al.
**摘要**：体外实验表明，重组CKB通过促进肌酸-磷酸肌酸循环增强癌细胞的能量代谢，提示其作为肿瘤治疗潜在靶点的可能性。
4. **文献名称**：*Application of Recombinant CKB in Neurodegenerative Disease Models*
**作者**：Chen L. et al.
**摘要**：在小鼠模型中，重组C蛋白显示出缓解神经能量代谢障碍的潜力，可能为阿尔茨海默病等疾病提供治疗策略。
---
**备注**：以上文献为示例，实际文献需通过PubMed、Web of Science等数据库检索关键词(如“CKB recombinant protein”、“Creatine Kinase Brain-type expression”)获取。建议结合具体研究方向筛选近年研究。
**Background of CKB Recombinant Protein**
Creatine kinase B-type (CKB), a key enzyme in cellular energy metabolism, catalyzes the reversible transfer of phosphate groups between creatine and ATP to maintain energy homeostasis, particularly in tissues with high energy demands like the brain, muscle, and heart. The CKB protein is encoded by the *CKB* gene and functions as a dimer, often pairing with another creatine kinase isoform (e.g., CK-M or CK-Mi) to form active complexes. Dysregulation of CKB expression or activity has been linked to various pathological conditions, including cancer, neurodegenerative diseases, and cardiovascular disorders, highlighting its biological and clinical relevance.
Recombinant CKB protein is produced using genetic engineering techniques, typically by expressing the *CKB* gene in heterologous systems such as *E. coli* or mammalian cell cultures. This approach ensures high purity, consistency, and scalability, addressing limitations of native protein extraction. Recombinant CKB retains enzymatic activity and structural integrity, making it a valuable tool for biochemical studies, drug screening, and diagnostic applications. For instance, it serves as a critical reagent in assays measuring creatine kinase activity to diagnose myocardial infarction or muscle damage.
In research, recombinant CKB aids in elucidating mechanisms of energy dysregulation in diseases. Recent studies also explore its role in tumor microenvironment adaptation, where cancer cells exploit CKB-mediated ATP regeneration to sustain proliferation under hypoxic conditions. Furthermore, recombinant CKB is pivotal in developing targeted therapies, such as inhibitors to disrupt cancer metabolic pathways or enhancers to mitigate neurodegenerative energy deficits.
Overall, CKB recombinant protein bridges basic science and translational applications, offering insights into cellular energetics and therapeutic opportunities across diverse diseases.
×
