| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UTS2R |
| Uniprot No | Q9UKP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MALTPESPSSFPGLAATGSSVPEPPGGPNATLNSSWASPTEPSSLEDLVATGTIGTLLSAMGVVGVVGNAYTLVVTCRSLRAVASMYVYVVNLALADLLYLLSIPFIVATYVTKEWHFGDVGCRVLFGLDFLTMHASIFTLTVMSSERYAAVLRPLDTVQRPKGYRKLLALGTWLLALLLTLPVMLAMRLVRRGPKSLCLPAWGPRAHRAYLTLLFATSIAGPGLLIGLLYARLARAYRRSQRASFKRARRPGARALRLVLGIVLLFWACFLPFWLWQLLAQYHQAPLAPRTARIVNYLTTCLTYGNSCANPFLYTLLTRNYRDHLRGRVRGPGSGGGRGPVPSLQPRARFQRCSGRSLSSCSPQPTDSLVLAPAAPARPAPEGPRAPA |
| 预测分子量 | 42,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UTS2R(Urotensin II受体)重组蛋白研究的模拟参考文献示例(文献为虚构，仅供格式参考)：
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1. **文献名称**: "Cloning and Functional Characterization of Recombinant Human UTS2R in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 本研究通过基因克隆技术成功在HEK293细胞中表达重组人源UTS2R，证实其与Urotensin II的高亲和力结合，并揭示了该受体激活后通过Gq蛋白介导的钙信号通路机制。
2. **文献名称**: "Structural Insights into UTS2R Activation by Cryo-EM Analysis"
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜技术解析了重组UTS2R与Urotensin II复合物的三维结构，揭示了配体结合口袋的关键氨基酸残基及受体激活的构象变化，为靶向药物设计提供结构基础。
3. **文献名称**: "Role of Recombinant UTS2R in Pulmonary Hypertension: In Vitro and In Vivo Studies"
**作者**: Tanaka K, et al.
**摘要**: 通过重组UTS2R蛋白模型发现，受体过表达加剧肺动脉高压病理进程，拮抗剂SB-710411显著抑制UTS2R信号通路，提示其作为治疗靶点的潜力。
4. **文献名称**: "UTS2R Recombinant Protein as a Biomarker in Hepatocellular Carcinoma"
**作者**: Wang X, et al.
**摘要**: 研究发现肝癌组织中重组UTS2R蛋白表达水平异常升高，且与血管生成标志物VEGF呈正相关，提示其可能作为肝癌诊断或预后的生物标志物。
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注：以上文献及作者为模拟内容，实际研究中需通过PubMed、Web of Science等数据库检索真实文献。
UTS2R (Urotensin II Receptor), a class A G protein-coupled receptor (GPCR), plays a critical role in mediating cellular responses to urotensin II (UII), a cyclic neuropeptide evolutionarily conserved across vertebrates. Initially identified in fish, the UII-UTS2R system regulates diverse physiological processes, including cardiovascular function, electrolyte balance, and stress responses. UTS2R activation triggers intracellular signaling pathways such as calcium mobilization, MAPK activation, and ERK phosphorylation, influencing vascular tone, cardiomyocyte contractility, and inflammatory responses.
Structurally, UTS2R contains seven transmembrane domains characteristic of GPCRs, with extracellular loops critical for UII binding. Its overexpression has been linked to pathological conditions like hypertension, atherosclerosis, heart failure, and metabolic disorders, making it a therapeutic target. However, the receptor's complex ligand interactions and tissue-specific signaling have hindered drug development.
Recombinant UTS2R protein, typically produced in mammalian or insect cell systems using genetic engineering, enables detailed biochemical and pharmacological studies. By expressing UTS2R in heterologous systems (e.g., HEK293 cells), researchers can isolate receptor-ligand binding dynamics, screen potential antagonists, and map signaling cascades without interference from endogenous receptors. Tagged versions (e.g., FLAG or His-tag) facilitate purification and structural analysis via crystallography or cryo-EM.
Recent studies utilize recombinant UTS2R to investigate biased signaling mechanisms and design subtype-selective modulators. Its role in cancer progression and fibrotic diseases has also emerged, expanding its biomedical relevance. Despite challenges in resolving its full activation mechanism, recombinant UTS2R remains vital for deciphering UII-mediated pathophysiology and advancing targeted therapies.
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