| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UST2 |
| Uniprot No | P00401 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534aa |
| 氨基酸序列 | MVQRWLYSTNAKDIAVLYFMLAIFSGMAGTAMSLIIRLELAAPGSQYLHGNSQLFNVLVVGHAVLMIFFLVMPALIGGFGNYLLPLMIGATDTAFPRINNIAFWVLPMGLVCLVTSTLVESGAGTGWTVYPPLSSIQAHSGPSVDLAIFALHLTSISSLLGAINFIVTTLNMRTNGMTMHKLPLFVWSIFITAFLLLLSLPVLSAGITMLLLDRNFNTSFFEVSGGGDPILYEHLFWFFGHPEVYILIIPGFGIISHVVSTYSKKPVFGEISMVYAMASIGLLGFLVWSHHMYIVGLDADTRAYFTSATMIIAIPTGIKIFSWLATIHGGSIRLATPMLYAIAFLFLFTMGGLTGVALANASLDVAFHDTYYVVGHFHYVLSMGAIFSLFAGYYYWSPQILGLNYNEKLAQIQFWLIFIGANVIFFPMHFLGINGMPRRIPDYPDAFAGWNYVASIGSFIATLSLFLFIYILYDQLVNGLNNKVNNKSVIYNKAPDFVESNTIFNLNTVKSSSIEFLLTSPPAVHSFNTPAVQS |
| 预测分子量 | 58,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UST2重组蛋白的3篇模拟参考文献，基于常见研究方向整理而成：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant UST2 in Cancer Cell Lines*
**作者**：Zhang Y. et al. (2020)
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组UST2蛋白，体外实验表明其能显著抑制多种癌细胞(如肺癌A549和乳腺癌MCF-7)的增殖，可能通过下调PI3K/AKT信号通路诱导细胞凋亡。
2. **文献名称**：*UST2 Recombinant Protein Attenuates Pulmonary Fibrosis via TGF-β Signaling Pathway*
**作者**：Li J. et al. (2018)
**摘要**：研究利用小鼠肺纤维化模型，发现注射重组UST2蛋白可减少胶原沉积和炎症反应，机制涉及抑制TGF-β1/Smad3通路的激活，提示UST2在抗纤维化治疗中的潜力。
3. **文献名称**：*Production and Purification of Recombinant UST2 in E. coli: Optimization and Functional Assays*
**作者**：Kim S. et al. (2019)
**摘要**：文章优化了UST2重组蛋白在大肠杆菌中的表达条件(如诱导温度、IPTG浓度)，并通过镍柱亲和层析高效纯化，最终产物经ELISA和圆二色谱验证具有高纯度及正确构象。
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**注**：以上为模拟参考文献，实际研究中建议通过PubMed、Google Scholar等平台以“UST2 recombinant protein”、“Urotensin II-related peptide”等关键词检索最新文献。
**Background of UST2 Recombinant Protein**
UST2 (Ubiquitin-like protein-specific protease 2), also known as USP2. is a member of the ubiquitin-specific protease family, which plays a critical role in regulating protein degradation via the ubiquitin-proteasome system. This system is essential for maintaining cellular homeostasis by selectively tagging proteins with ubiquitin for proteasomal breakdown. USP2 functions as a deubiquitinating enzyme (DUB), removing ubiquitin chains from target proteins to stabilize them or modulate their activity.
Structurally, USP2 contains a conserved catalytic domain responsible for its protease activity. Alternative splicing generates multiple isoforms, with USP2-69 and USP2-45 being the most studied. These isoforms exhibit distinct tissue expression patterns and substrate specificities. For instance, USP2-45 is highly expressed in the liver and kidney, while USP2-69 is prominent in the brain and testis.
Research highlights USP2's involvement in critical cellular processes, including cell cycle regulation, apoptosis, and oncogenesis. It interacts with substrates such as MDM2 (a negative regulator of tumor suppressor p53), fatty acid synthase (FASN), and cyclin D1. influencing pathways like p53 signaling, lipid metabolism, and cell proliferation. Dysregulation of USP2 has been linked to cancers (e.g., prostate, liver, and glioblastoma), where it often acts as an oncogene by stabilizing pro-survival proteins.
The recombinant UST2 protein, typically produced in *E. coli* or mammalian expression systems, retains enzymatic activity and is widely used *in vitro* to study deubiquitination mechanisms, screen inhibitors, or explore therapeutic strategies targeting USP2-associated diseases. Its role in metabolic disorders and neurodegenerative conditions is also under investigation, underscoring its multifaceted biological relevance.
In summary, USP2 recombinant protein serves as a vital tool for dissecting ubiquitination dynamics and developing targeted therapies, particularly in oncology and metabolic research.
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