| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM5 |
| Uniprot No | P33992 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-734aa |
| 氨基酸序列 | SGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ ELPDAVPHGE MPRHMQLYCD RYLCDKVVPG NRVTIMGIYS IKKFGLTTSR GRDRVGVGIR SSYIRVLGIQ VDTDGSGRSF AGAVSPQEEE EFRRLAALPN VYEVISKSIA PSIFGGTDMK KAIACLLFGG SRKRLPDGLT RRGDINLLML GDPGTAKSQL LKFVEKCSPI GVYTSGKGSS AAGLTASVMR DPSSRNFIME GGAMVLADGG VVCIDEFDKM REDDRVAIHE AMEQQTISIA KAGITTTLNS RCSVLAAANS VFGRWDETKG EDNIDFMPTI LSRFDMIFIV KDEHNEERDV MLAKHVITLH VSALTQTQAV EGEIDLAKLK KFIAYCRVKC GPRLSAEAAE KLKNRYIIMR SGARQHERDS DRRSSIPITV RQLEAIVRIA EALSKMKLQP FATEADVEEA LRLFQVSTLD AALSGTLSGV EGFTSQEDQE MLSRIEKQLK RRFAIGSQVS EHSIIKDFTK QKYPEHAIHK VLQLMLRRGE IQHRMQRKVL YRLK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCM5重组蛋白的3篇参考文献及其摘要概述：
---
1. **文献名称**: *"Expression and purification of recombinant human MCM5 for functional studies"*
**作者**: Smith J, et al.
**摘要**: 该研究描述了通过大肠杆菌表达系统高效表达人源重组MCM5蛋白的优化方法，并利用亲和层析技术纯化获得高纯度蛋白。作者验证了重组MCM5在体外结合ATP的能力，并证明其与其他MCM复合物成员的相互作用，为后续功能研究奠定基础。
---
2. **文献名称**: *"Structural insights into the MCM5 protein in DNA replication initiation"*
**作者**: Tanaka H, et al.
**摘要**: 通过冷冻电镜解析重组MCM5蛋白与DNA结合的复合物结构，揭示了MCM5在DNA解旋酶核心复合物中的构象变化及其在复制起始阶段的关键作用。研究还发现重组MCM5的N端结构域对复合物组装至关重要。
---
3. **文献名称**: *"Development of an MCM5-based ELISA for early cancer detection"*
**作者**: Chen L, et al.
**摘要**: 利用重组MCM5蛋白作为抗原，开发了一种高灵敏度ELISA检测方法，用于检测尿液或血液中MCM5抗体水平。临床数据显示其在膀胱癌和宫颈癌早期筛查中的潜在价值，证明重组MCM5作为诊断标志物的可行性。
---
(注：以上文献为示例性内容，实际引用需根据具体研究补充真实文献信息。)
**Background of MCM5 Recombinant Protein**
The mini-chromosome maintenance protein 5 (MCM5) is a critical member of the MCM family, a group of six conserved proteins (MCM2-7) essential for DNA replication in eukaryotic cells. These proteins form a hexameric helicase complex that unwinds DNA during the initiation and elongation phases of replication, ensuring faithful genome duplication. MCM5. encoded by the *MCM5* gene, plays a pivotal role in assembling and stabilizing this helicase complex, as well as regulating its ATPase activity. Dysregulation of MCM5 is linked to genomic instability and has been implicated in various cancers, making it a potential biomarker for cancer diagnosis and prognosis.
Recombinant MCM5 protein is produced via molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cell lines), enabling large-scale purification for functional studies. Its recombinant form retains structural and enzymatic properties, facilitating investigations into DNA replication mechanisms, cell cycle regulation, and interactions with replication-associated factors like Cdc45 and GINS.
Research on MCM5 recombinant protein has advanced understanding of replication licensing, checkpoint control, and oncogenic pathways. It is also utilized in drug discovery to identify inhibitors targeting helicase activity for anticancer therapies. Additionally, MCM5-specific antibodies generated from recombinant protein are employed in diagnostic assays to detect dysregulated expression in precancerous or malignant tissues.
Overall, MCM5 recombinant protein serves as a vital tool for dissecting DNA replication biology and developing translational applications in oncology and molecular diagnostics.
×
