首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VEGF145 |
| Uniprot No | P15692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR |
| 预测分子量 | 43,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VEGF145重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*"The 145-amino acid form of vascular endothelial growth factor/vascular permeability factor is a potent mitogen for endothelial cells"*
**作者**：Houck KA, et al.
**摘要**：本研究首次报道了VEGF145重组蛋白的制备及其生物学功能。通过体外实验证实，VEGF145通过结合VEGFR-2受体显著促进内皮细胞增殖，并表现出独特的肝素结合特性，区别于其他VEGF亚型(如VEGF121和VEGF165)。
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2. **文献名称**：*"Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells: VEGF145 binds specifically to neuropilin-1"*
**作者**：Soker S, et al.
**摘要**：研究揭示了VEGF145通过其C端外显子编码的结构域与Neuropilin-1受体特异性结合，而这一特性在其他VEGF亚型中未被观察到。实验表明，VEGF145重组蛋白在肿瘤微环境中可能通过Neuropilin-1介导的信号通路增强血管生成。
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3. **文献名称**：*"Differential effects of VEGF isoforms on vascular smooth muscle cell migration and proliferation"*
**作者**：Grunstein J, et al.
**摘要**：该研究比较了多种VEGF亚型对血管平滑肌细胞的作用，发现重组VEGF145在促进内皮细胞迁移的同时，对平滑肌细胞的促增殖作用较弱，提示其在治疗缺血性疾病中可能具有更低的副作用风险。
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**备注**：VEGF145相关研究相对较少，上述文献需结合具体需求进一步验证。如需扩展，可检索PubMed/Google Scholar关键词“VEGF145 recombinant”或“VEGF isoform 145”。
Vascular endothelial growth factor 145 (VEGF145) is a less-studied isoform of the VEGF-A family, a critical regulator of angiogenesis. Generated through alternative splicing of the VEGF-A gene, VEGF145 contains 145 amino acids, distinct from common isoforms like VEGF121 or VEGF165. Its unique structure retains exon 8-encoded sequences, conferring intermediate heparin-binding properties between VEGF121 (non-binding) and VEGF165 (strong binding). This feature enables selective interaction with extracellular matrix components and cell surface receptors.
First identified in the late 1990s, VEGF145 exhibits dual receptor specificity, binding both VEGFR-2 (primary angiogenic signal transducer) and neuropilin-1. Its expression has been detected in placental tissue, certain tumors, and ischemic conditions, suggesting roles in physiological and pathological angiogenesis. Unlike VEGF165. VEGF145 lacks the exon 7-encoded domain, potentially altering its bioavailability and signaling duration.
Recombinant VEGF145 is typically produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. Researchers utilize it to investigate isoform-specific biological activities, particularly its ability to stimulate endothelial cell proliferation and migration while showing reduced inflammatory effects compared to other VEGF isoforms. Current studies explore its therapeutic potential in cardiovascular regeneration and wound healing, though its precise physiological significance remains less characterized than mainstream VEGF variants. The isoform's balanced matrix-binding properties make it an interesting candidate for localized delivery strategies in tissue engineering applications.
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