| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM5C |
| Uniprot No | Q76B58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-766aa |
| 氨基酸序列 | AVSDQHA TSPFDWLLSD KGPFHRSQEY TDFVDRSRQG FSTRYKIYRE FGRWKVNNLA VERRNFLGSP LPLAPEFFRN IRLLGRRPTL QQITENLIKK YGTHFLLSAT LGGEESLTIF VDKRKLSKRA EGSDSTTNSS SVTLETLHQL AASYFIDRDS TLRRLHHIQI ASTAIKVTET RTGPLGCSNY DNLDSVSSVL VQSPENKIQL QGLQVLLPDY LQERFVQAAL SYIACNSEGE FICKENDCWC HCGPKFPECN CPSMDIQAME ENLLRITETW KAYNSDFEES DEFKLFMKRL PMNYFLNTST IMHLWTMDSN FQRRYEQLEN SMKQLFLKAQ KIVHKLFSLS KRCHKQPLIS LPRQRTSTYW LTRIQSFLYC NENGLLGSFS EETHSCTCPN DQVVCTAFLP CTVGDASACL TCAPDNRTRC GTCNTGYMLS QGLCKPEVAE STDHYIGFET DLQDLEMKYL LQKTDRRIEV HAIFISNDMR LNSWFDPSWR KRMLLTLKSN KYKSSLVHMI LGLSLQICLT KNSTLEPVLA VYVNPFGGSH SESWFMPVNE NSFPDWERTK LDLPLQCYNW TLTLGNKWKT FFETVHIYLR SRIKSNGPNG NESIYYEPLE FIDPSRNLGY MKINNIQVFG YSMHFDPEAI RDLILQLDYP YTQGSQDSAL LQLLEIRDRV NKLSPPGQRR LDLFSCLLRH RLKLSTSEVV RIQSALQAFN AKLPNTMDYD TTKLCS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM5C重组蛋白的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *FAM5C Recombinant Protein Suppresses Tumor Growth by Modulating Angiogenesis in Colorectal Cancer*
**作者**: Tanaka K. et al.
**摘要**: 本研究通过大肠杆菌表达系统制备了重组FAM5C蛋白，并发现其通过抑制VEGF信号通路显著减少结直肠癌小鼠模型中的肿瘤血管生成，提示FAM5C可能作为抗血管生成治疗的潜在靶点。
2. **文献名称**: *Expression and Functional Analysis of Recombinant FAM5C in Neurodegenerative Models*
**作者**: Smith J. et al.
**摘要**: 作者在HEK293细胞中表达了重组FAM5C蛋白，并证明其能通过激活PI3K/AKT通路减少β-淀粉样蛋白诱导的神经元凋亡，提示FAM5C在阿尔茨海默病中的保护作用。
3. **文献名称**: *FAM5C Recombinant Protein Enhances Insulin Sensitivity in Adipocytes via AMPK Activation*
**作者**: Chen L. et al.
**摘要**: 该研究利用哺乳动物表达系统纯化FAM5C重组蛋白，发现其通过激活AMPK通路改善脂肪细胞的胰岛素敏感性，为代谢综合征的治疗提供了新思路。
4. **文献名称**: *Structural Insights into FAM5C Recombinant Protein and Its Interaction with Inflammatory Cytokines*
**作者**: Müller R. et al.
**摘要**: 通过X射线晶体学解析重组FAM5C蛋白结构，揭示其与TNF-α的结合位点，表明FAM5C可能通过调控炎症因子参与慢性炎症性疾病。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索确认。建议使用关键词“FAM5C recombinant protein”或“FAM5C expression”查找最新研究。
**Background of FAM5C Recombinant Protein**
FAM5C (Family with sequence similarity 5 member C), also known as *BRINP3* (BMP/retinoic acid-inducible neural-specific protein 3) or *FAM5C1*, is a secreted protein encoded by the *FAM5C* gene located on human chromosome 1p36.22. It belongs to the FAM5 protein family, which shares conserved domains implicated in cell adhesion, neuronal development, and tissue homeostasis. Structurally, FAM5C contains multiple thrombospondin type 1 repeats (TSRs), which are associated with extracellular matrix interactions and cell signaling.
Functionally, FAM5C is highly expressed in the brain, particularly in neurons, and plays roles in neural differentiation, synaptic plasticity, and neuroprotection. Studies suggest its involvement in regulating neurogenesis and apoptosis, potentially linked to neurodegenerative disorders like Alzheimer’s disease. Beyond the nervous system, FAM5C is associated with cardiovascular health, where it may influence endothelial cell function and atherosclerosis progression. Additionally, it has been implicated in cancer biology, showing both tumor-suppressive and oncogenic effects depending on context, such as modulating cell proliferation and metastasis in gastric or colorectal cancers.
Recombinant FAM5C protein is engineered using expression systems like mammalian cells or *E. coli* to ensure proper folding and post-translational modifications. It serves as a critical tool for *in vitro* and *in vivo* studies to dissect its molecular mechanisms, including ligand-receptor interactions and signaling pathways (e.g., MAPK/ERK). Researchers also utilize it for antibody development, biomarker discovery, and therapeutic exploration, particularly in neurodegenerative and cardiovascular diseases.
Despite progress, FAM5C’s full biological significance remains under investigation, with ongoing studies aiming to clarify its dual roles in different tissues and diseases, as well as its potential as a diagnostic or therapeutic target.
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