| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGTR2 |
| Uniprot No | P50052 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363 aa |
| 氨基酸序列 | MKGNSTLATTSKNITSGLHFGLVNISGNNESTLNCSQKPSDKHLDAIPILYYIIFVIGFLVNIVVVTLFCCQKGPKKVSSIYIFNLAVADLLLLATLPLWATYYSYRYDWLFGPVMCKVFGSFLTLNMFASIFFITCMSVDRYQSVIYPFLSQRRNPWQASYIVPLVWCMACLSSLPTFYFRDVRTIEYLGVNACIMAFPPEKYAQWSAGIALMKNILGFIIPLIFIATCYFGIRKHLLKTNSYGKNRITRDQVLKMAAAVVLAFIICWLPFHVLTFLDALAWMGVINSCEVIAVIDLALPFAILLGFTNSCVNPFLYCFVGNRFQQKLRSVFRVPITWLQGKRESMSCRKSSSLREMETFVS |
| 预测分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGTR2重组蛋白研究的3篇代表性文献示例(注：文献为示例性格式，具体内容需根据实际检索结果调整)：
1. **标题**："Expression and Functional Characterization of Recombinant Human AGTR2 in Mammalian Cells"
**作者**：Smith A, et al.
**摘要**：研究通过哺乳动物表达系统成功表达并纯化AGTR2重组蛋白，证实其与血管紧张素II结合的能力，并解析其在细胞信号通路中的作用。
2. **标题**："Structural Insights into AGTR2 Activation via Cryo-EM Analysis of Recombinant Protein Complexes"
**作者**：Zhang L, et al.
**摘要**：利用冷冻电镜技术解析重组AGTR2蛋白与配体结合的分子结构，揭示受体激活的构象变化机制，为靶向药物设计提供依据。
3. **标题**："AGTR2 Recombinant Protein Attenuates Fibrosis in a Mouse Model of Cardiac Hypertrophy"
**作者**：Tanaka K, et al.
**摘要**：通过动物实验证明重组AGTR2蛋白可通过调控TGF-β通路抑制心肌纤维化，提示其在心血管疾病治疗中的潜在应用价值。
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如需获取真实文献，建议在PubMed或Web of Science中检索关键词：**"AGTR2 recombinant protein" + "expression"/"structure"/"function"**，并筛选近5年高被引论文。
The angiotensin II receptor type 2 (AGTR2) is a G protein-coupled receptor (GPCR) that binds angiotensin II, a key peptide hormone in the renin-angiotensin system (RAS). While its counterpart, AGTR1. is widely studied for roles in vasoconstriction, blood pressure regulation, and cardiovascular pathologies, AGTR2 has emerged as a modulator with distinct, often opposing functions. Predominantly expressed during fetal development, AGTR2 is found in limited adult tissues, including the kidneys, adrenal glands, brain, and reproductive organs. It is implicated in vasodilation, anti-proliferative effects, tissue repair, and apoptosis, suggesting a counter-regulatory role to AGTR1.
Recombinant AGTR2 protein is engineered using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications and membrane localization. The protein often includes tags (e.g., FLAG, His-tag) for purification and detection. Researchers use it to study RAS signaling, particularly in pathologies such as hypertension, heart failure, renal disease, and neuroinflammation. AGTR2 activation is linked to nitric oxide release, kinase pathways (e.g., MAPK), and interactions with cellular scaffolds, offering insights into its protective mechanisms.
Recent studies highlight AGTR2's potential therapeutic applications, including mitigating organ fibrosis, enhancing post-injury tissue regeneration, and modulating oxidative stress. However, its complex signaling crosstalk and tissue-specific effects necessitate further exploration. Recombinant AGTR2 serves as a vital tool for structural studies, drug screening, and deciphering its enigmatic role in health and disease.
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