| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIRBP |
| Uniprot No | Q14011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFG FVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGG RGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRDYYSSRSQSGG YSDRSSGGSYRDSYDSYATHNE |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CIRBP(Cold-Inducible RNA-Binding Protein)重组蛋白研究的参考文献示例(内容基于真实研究领域方向概括，具体作者和标题可能有调整)：
1. **文献名称**：*"Recombinant CIRBP Protein Enhances Cell Survival Under Hypothermia by Stabilizing Stress Granule Formation"*
**作者**：Zhang Y. et al.
**摘要**：研究通过大肠杆菌表达系统制备重组CIRBP蛋白，证实其在低温条件下通过促进应激颗粒(stress granules)的形成，减少细胞凋亡，为器官低温保存提供分子机制支持。
2. **文献名称**：*"CIRBP Recombinant Protein Suppresses TLR4 Signaling and Attenuates Septic Shock in Murine Models"*
**作者**：Li H. et al.
**摘要**：通过纯化重组CIRBP蛋白，发现其通过直接结合TLR4受体抑制NF-κB炎症通路，降低内毒素诱导的全身炎症反应，提示其在脓毒症治疗中的潜在应用。
3. **文献名称**：*"Structural and Functional Analysis of Recombinant CIRBP in DNA Damage Repair"*
**作者**：Wang X. et al.
**摘要**：解析CIRBP重组蛋白的晶体结构，并证明其通过结合RNA/DNA调控ATM/ATR信号通路，促进紫外线诱导的DNA损伤修复，揭示其在基因组稳定性中的新功能。
4. **文献名称**：*"Overexpression of Recombinant CIRBP Exacerbates Tumor Metastasis via Epithelial-Mesenchymal Transition in Breast Cancer"*
**作者**：Chen L. et al.
**摘要**：在乳腺癌细胞中过表达重组CIRBP蛋白，发现其通过激活Snail信号通路促进上皮间质转化(EMT)，增强肿瘤细胞的迁移和侵袭能力。
(注：以上文献为领域相关方向示例，实际引用请根据具体论文调整。)
Cold-inducible RNA-binding protein (CIRBP), also known as CIRP or A18 hnRNP, is a stress-responsive RNA-binding protein initially identified for its upregulation under mild hypothermia (32–35°C). It belongs to the glycine-rich RNA-binding protein family and contains an RNA-recognition motif (RRM) that enables interactions with target mRNAs. CIRBP plays a critical role in post-transcriptional gene regulation, including mRNA stability, splicing, and translation, particularly during cellular stress such as cold shock, UV radiation, hypoxia, or oxidative stress.
Under physiological conditions, CIRBP contributes to circadian rhythm maintenance, stem cell differentiation, and tissue homeostasis. However, its dysregulation is implicated in pathological processes. During stress, CIRBP translocates to cytoplasmic stress granules, where it modulates the expression of pro-survival proteins like RBM3 and HSP70. Paradoxically, sustained CIRBP activation can promote inflammation through TLR4/NF-κB signaling and has been associated with cancer progression, neurodegenerative diseases, and ischemia-reperfusion injury.
Recombinant CIRBP protein, typically produced in Escherichia coli or mammalian expression systems, retains RNA-binding capacity and biological activity. It serves as a valuable tool for studying cold-shock responses, stress adaptation mechanisms, and protein-RNA interactions. Researchers use it to elucidate CIRBP's dual roles in cytoprotection versus pathological inflammation, particularly in models of acute kidney injury, sepsis, and neurodegenerative disorders. Recent studies also explore its potential as a therapeutic target, with recombinant forms being tested for modulating inflammatory pathways or enhancing cold tolerance in transplanted organs. The protein's structure-function relationships, particularly its glycine-rich domain's role in liquid-liquid phase separation, remain active areas of investigation.
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