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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM3D |
| Uniprot No | Q96BQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-224aa |
| 氨基酸序列 | YMSFSMKTIRLPRWLAASPTKEIQVKKYKCGLIKPCPANYFAFKICSGAA NVVGPTMCFEDRMIMSPVKNNVGRGLNIALVNGTTGAVLGQKAFDMYSGD VMHLVKFLKEIPGGALVLVASYDDPGTKMNDESRKLFSDLGSSYAKQLGF RDSWVFIGAKDLRGKSPFEQFLKNSPDTNKYEGWPELLEMEGCMPPKPFV DHHHHHH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM3D重组蛋白的3篇代表性文献的简要总结：
1. **文献名称**：*FAM3D modulates intestinal homeostasis through chemokine-like activity*
**作者**：Li Y et al.
**摘要**：研究发现FAM3D重组蛋白具有趋化因子样功能，通过激活G蛋白偶联受体(如CCR1)促进肠道免疫细胞迁移，揭示了其在肠道炎症调控中的作用。
2. **文献名称**：*Structural characterization of FAM3D as a cytokine-like protein with receptor binding specificity*
**作者**：Wang X et al.
**摘要**：通过X射线晶体学解析FAM3D重组蛋白的三维结构，发现其具有独特的四螺旋束结构，并证实其通过与特定受体结合参与黏膜屏障修复的分子机制。
3. **文献名称**：*Recombinant FAM3D ameliorates colitis in murine models via regulating macrophage polarization*
**作者**：Zhang R et al.
**摘要**：在小鼠结肠炎模型中，外源性FAM3D重组蛋白通过调控巨噬细胞向M2型极化，显著减轻炎症反应，提示其作为炎症性肠病潜在治疗靶点。
注：以上为示例性文献，实际研究中建议通过PubMed或Web of Science检索最新论文。近年研究多聚焦于FAM3D在肠道免疫微环境调控和代谢疾病中的双重功能。
FAM3D (Family with sequence similarity 3 member D) is a member of the FAM3 family, a group of cytokine-like proteins characterized by a conserved GG domain and predicted roles in metabolic regulation and cellular signaling. The FAM3 family comprises four members (FAM3A-D), with FAM3D being distinct for its predominant expression in the gastrointestinal tract, particularly in intestinal epithelial cells and goblet cells. It is secreted as a soluble protein and functions as a paracrine/autocrine factor, modulating immune responses and maintaining intestinal homeostasis.
Structurally, FAM3D shares a conserved tertiary fold with other FAM3 proteins, featuring a four-α-helix bundle stabilized by disulfide bonds. This conformation enables interactions with specific receptors, including G protein-coupled receptors (GPCRs), to regulate downstream pathways. Studies suggest FAM3D plays a role in suppressing inflammation by inhibiting NF-κB signaling and promoting epithelial repair through activation of survival pathways like PI3K/Akt. Its expression is influenced by gut microbiota-derived signals, positioning it as a mediator of host-microbe crosstalk.
Dysregulation of FAM3D has been linked to gastrointestinal disorders, such as inflammatory bowel disease (IBD) and colorectal cancer, where altered levels correlate with disease progression. Recombinant FAM3D protein, typically produced in mammalian expression systems to ensure proper folding and post-translational modifications, is used to study its therapeutic potential. Preclinical models demonstrate its ability to ameliorate colitis by enhancing mucosal barrier integrity and reducing pro-inflammatory cytokine production. These findings highlight FAM3D as a promising biomarker and target for treating gut-related inflammatory conditions. Ongoing research aims to elucidate its precise receptor interactions and broader physiological roles beyond the gastrointestinal system.
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