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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM3B |
| Uniprot No | P58499 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-235aa |
| 氨基酸序列 | MKHHHHHHAS ELIPDAPLSS AAYSIRSIGE RPVLKAPVPK RQKCDHWTPC PSDTYAYRLL SGGGRSKYAK ICFEDNLLMG EQLGNVARGI NIAIVNYVTG NVTATRCFDM YEGDNSGPMT KFIQSAAPKS LLFMVTYDDG STRLNNDAKN AIEALGSKEI RNMKFRSSWV FIAAKGLELP SEIQREKINH SDAKNNRYSG WPAEIQIEGC IPKERS |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FAM3B重组蛋白的关键文献摘要(虚构示例，仅供格式参考)：
1. **"FAM3B/PANDER: A Novel Regulator of Hepatic Glucose Production"**
- 作者：Zhang L, Yang JK et al.
- 摘要：本研究首次报道了重组FAM3B蛋白(又称PANDER)通过激活PI3K/Akt信号通路抑制肝细胞糖异生，揭示了其在2型糖尿病中调控血糖稳态的潜在机制。
2. **"Crystal Structure of Recombinant FAM3B Reveals a Cytokine-like Fold"**
- 作者：Chen X, Wang H et al.
- 摘要：通过X射线晶体学解析了重组FAM3B蛋白的三维结构，发现其具有类似细胞因子的结构特征，为研究其与胰岛素分泌相关的分子互作提供了结构基础。
3. **"FAM3B Recombinant Protein Induces Pancreatic β-cell Apoptosis via Mitochondrial Pathway"**
- 作者：Li Y, Liu Q et al.
- 摘要：体外实验表明，重组FAM3B蛋白通过激活线粒体凋亡通路(如Caspase-3/9)促进胰岛β细胞凋亡，提示其在糖尿病β细胞功能障碍中的病理作用。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar以关键词 **"FAM3B recombinant"、"PANDER protein"** 检索获取。
**Background of FAM3B Recombinant Protein**
FAM3B, belonging to the Family with Sequence Similarity 3 (FAM3) group, is a cytokine-like protein encoded by the *FAM3B* gene located on human chromosome 21q22.3. Initially identified in 2002. FAM3B gained attention due to its pancreatic expression and potential role in glucose metabolism and diabetes pathogenesis. The protein is also termed PANcreatic DERived factor (PANDER), reflecting its primary secretion from pancreatic α- and β-cells. Structurally, FAM3B comprises 224 amino acids, featuring four α-helical domains characteristic of the FAM3 family, but lacks homology to known cytokines or hormones.
Research highlights FAM3B as a regulator of β-cell function and survival. It is co-secreted with insulin under high-glucose conditions, suggesting interplay in glucose homeostasis. However, prolonged FAM3B exposure induces β-cell apoptosis via caspase-3 activation, linking it to β-cell dysfunction in type 2 diabetes (T2D). Animal studies show that FAM3B overexpression impairs glucose tolerance, while its inhibition enhances insulin sensitivity, underscoring dual roles in metabolic regulation.
Recombinant FAM3B protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies. Purification often involves affinity chromatography, yielding bioactive forms for *in vitro* and *in vivo* assays. Investigations using recombinant FAM3B have elucidated its signaling mechanisms, including interactions with mitochondrial proteins and modulation of hepatic gluconeogenesis. Despite progress, its receptor remains unidentified, and downstream pathways (e.g., JAK/STAT involvement) require further validation.
FAM3B’s dual role as a metabolic modulator and apoptosis inducer positions it as a potential therapeutic target for diabetes. However, its complex biology warrants deeper exploration to reconcile conflicting observations and clarify its physiological versus pathological contributions.
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