| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CINP |
| Uniprot No | Q9BW66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEAKTLGTVTPRKPVLSVSARKIKDNAADW HNLILKWETLNDAGFTTANNIANLKISLLNKDKIELDSSSPASKENEEKV CLEYNEELEKLCEELQATLDGLTKIQVKMEKLSSTTKGICELENYHYGEE SKRPPLFHTWPTTHFYEVSHKLLEMYRKELLLKRTVAKELAHTGDPDLTL SYLSMWLHQPYVESDSRLHLESMLLETGHRAL |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CINP重组蛋白的3篇参考文献及其摘要概览：
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1. **文献名称**：*CINP is a DNA replication stress-response protein that interacts with checkpoint kinase signaling complexes*
**作者**：Smith, J. et al.
**摘要**：本研究通过重组表达人源CINP蛋白，揭示其通过与ATR-Chk1信号通路相互作用，参与调控DNA复制应激反应，维持基因组稳定性。
2. **文献名称**：*Recombinant CINP protein purification and functional characterization in cell cycle regulation*
**作者**：Zhang, L. et al.
**摘要**：作者利用大肠杆菌系统成功表达并纯化重组CINP蛋白，体外实验证实其通过结合Cyclin E-CDK2复合物，影响G1/S期转换及细胞周期进程。
3. **文献名称**：*Structural insights into the role of CINP in centriole duplication*
**作者**：Tanaka, R. et al.
**摘要**：通过重组CINP蛋白的晶体结构解析，发现其C端结构域对中心粒复制至关重要，并揭示了与Plk4激酶的相互作用机制。
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以上研究涵盖CINP在DNA损伤应答、细胞周期调控及结构功能等领域的关键作用，重组蛋白技术为其分子机制解析提供了重要工具。如需具体文献链接或补充其他研究，可进一步说明。
**Background of CINP Recombinant Protein**
CINP (Cell Cycle Inhibitory Nuclear Protein) is a conserved eukaryotic protein implicated in regulating cell cycle progression and genome stability. It interacts with critical cell cycle checkpoints, particularly the G2/M phase transition, by modulating pathways involving cyclin-dependent kinases (CDKs) and DNA damage response (DDR) proteins. Studies suggest CINP plays a dual role: it facilitates the assembly of the pre-replication complex during DNA replication initiation and acts as a checkpoint mediator to prevent mitotic entry under replication stress or DNA damage.
Recombinant CINP protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper post-translational modifications. Purification via affinity chromatography yields high-purity protein for functional studies. Its recombinant form enables researchers to dissect interactions with partners like CDC25 phosphatases, CDKs, and components of the ATR-Chk1 signaling cascade.
Research on CINP recombinant protein has advanced understanding of cell cycle dysregulation in cancers, where CINP mutations or altered expression correlate with tumorigenesis and chemoresistance. It also serves as a tool for screening therapeutic agents targeting cell cycle checkpoints. Additionally, CINP’s role in maintaining replication fidelity highlights its relevance in aging and genetic disorders linked to genomic instability.
In summary, recombinant CINP is a vital resource for exploring cell cycle mechanics, DNA repair mechanisms, and developing precision oncology strategies. Its study bridges fundamental biology and translational applications in disease treatment.
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