| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX9 |
| Uniprot No | P48436 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509aa |
| 氨基酸序列 | MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQE NTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSK NKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESE KRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISP NAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKA DLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPP NGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPP QAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTE QLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSS YYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQ PVYTQLTRP |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SOX9重组蛋白的3篇参考文献示例(注：文献为示例性概括，非真实存在)：
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1. **文献名称**：*Production and Functional Analysis of Recombinant SOX9 Protein in Bacterial Expression Systems*
**作者**：Zhang L, et al.
**摘要**：本研究报道了在大肠杆菌中高效表达并纯化SOX9重组蛋白的优化方法，通过亲和层析获得高纯度蛋白。功能实验表明，重组SOX9能够特异性结合软骨相关基因的DNA序列，并激活报告基因表达，验证了其转录活性。
2. **文献名称**：*SOX9 Recombinant Protein Enhances Chondrogenic Differentiation in Mesenchymal Stem Cells*
**作者**：Tanaka K, et al.
**摘要**：利用哺乳动物细胞表达系统制备SOX9重组蛋白，并证明其可通过激活Sox5/Sox6通路促进间充质干细胞向软骨细胞分化。研究为软骨再生治疗提供了潜在工具。
3. **文献名称**：*Structural Insights into SOX9-DNA Interactions Using Recombinant Protein Crystallography*
**作者**：Müller R, et al.
**摘要**：通过昆虫细胞系统表达SOX9重组蛋白，解析其与靶DNA复合物的晶体结构，揭示了SOX9结合DNA的关键结构域及突变对其功能的影响，为遗传性骨骼疾病的机制研究提供依据。
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以上文献摘要概括了SOX9重组蛋白在表达体系开发、分化调控及结构解析中的应用，涵盖基础研究与潜在临床价值。如需真实文献，建议通过PubMed或Web of Science检索具体关键词。
**Background of SOX9 Recombinant Protein**
SOX9 (SRY-related HMG-box 9) is a transcription factor belonging to the SOX family, which plays pivotal roles in embryonic development, cell differentiation, and tissue homeostasis. It is particularly critical for chondrogenesis, sex determination, and maintaining stem cell populations in various organs. Structurally, SOX9 contains a DNA-binding high-mobility group (HMG) domain that enables sequence-specific interactions with regulatory regions of target genes, such as *COL2A1* (collagen type II) and *ACAN* (aggrecan), which are essential for cartilage formation. Dysregulation of SOX9 is linked to developmental disorders (e.g., campomelic dysplasia) and diseases like osteoarthritis or cancer.
Recombinant SOX9 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian, or insect cells) to study its biochemical functions *in vitro*. Bacterial systems offer high yields but lack post-translational modifications, while eukaryotic systems provide properly folded, modified proteins closer to native forms. The purified protein is often tagged (e.g., His, GST) for ease of isolation and detection. Researchers utilize recombinant SOX9 to investigate its DNA-binding kinetics, interactions with co-regulators (e.g., β-catenin), or roles in directing stem cell differentiation into chondrocytes. It also serves as a tool in disease modeling, drug screening, and regenerative medicine strategies aimed at cartilage repair. Challenges in production include maintaining solubility and functional activity, often addressed through optimized expression conditions or fusion partners. Recent advances in structural biology and CRISPR-based editing further enhance its application in mechanistic studies and therapeutic development.
In summary, recombinant SOX9 is a vital resource for dissecting developmental pathways and exploring therapeutic interventions for SOX9-associated pathologies.
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