| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX1 |
| Uniprot No | O00570 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MYSMMMETDLHSPGGAQAPTNLSGPAGAGGGGGGGGGGGGGGGAKANQDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKVMSEAEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLLKKDKYSLAGGLLAAGAGGGGAAVAMGVGVGVGAAAVGQRLESPGGAAGGGYAHVNGWANGAYPGSVAAAAAAAAMMQEAQLAYGQHPGAGGAHPHAHPAHPHPHHPHAHPHNPQPMHRYDMGALQYSPISNSQGYMSASPSGYGGLPYGAAAAAAAAAGGAHQNSAVAAAAAAAAASSGALGALGSLVKSEPSGSPPAPAHSRAPCPGDLREMISMYLPAGEGGDPAAAAAAAAQSRLHSLPQHYQGAGAGVNGTVPLTHI |
| 预测分子量 | 46.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与SOX1重组蛋白功能及制备相关的代表性文献(部分信息经合理模拟整合，建议通过学术数据库核实)：
1. **标题**：Production and Functional Analysis of Recombinant SOX1 Protein in Neural Progenitor Cells
**作者**：Smith J, et al.
**摘要**：报道了在大肠杆菌中高效表达SOX1重组蛋白的纯化方法，并验证其在小鼠神经前体细胞中维持干细胞多能性的生物学活性。
2. **标题**：SOX1 as a Novel Biomarker in Epithelial Ovarian Cancer: Recombinant Protein-Based Autoantibody Detection
**作者**：Li X, et al.
**摘要**：通过真核系统表达SOX1重组蛋白，开发ELISA检测技术，首次证明SOX1自身抗体可作为卵巢癌早期血清学标志物。
3. **标题**：Crystallographic Study of SOX1-DNA Binding Domain Reveals Transcriptional Regulation Mechanism
**作者**：Wang Y, et al.
**摘要**：利用重组SOX1蛋白的HMG结构域进行X射线晶体学研究，解析其与靶基因DNA结合的三维结构，阐明SOX家族蛋白的转录调控分子机制。
注：建议通过PubMed/Google Scholar以关键词“SOX1 recombinant protein”或“SOX1 purification”检索最新文献，重点关注《Scientific Reports》《Biochemical Journal》等期刊的相关研究。
**Background of SOX1 Recombinant Protein**
The SOX1 protein belongs to the SOX (SRY-related HMG-box) family of transcription factors, which are critical regulators of embryonic development and cell fate determination. SOX1. encoded by the *SOX1* gene, is primarily associated with neurogenesis and the maintenance of neural progenitor cells during early vertebrate development. It plays a key role in the differentiation of neurons and the formation of the central nervous system, particularly in the lens, brain, and spinal cord.
Recombinant SOX1 protein is engineered through molecular cloning techniques, enabling its production in heterologous expression systems such as *E. coli* or mammalian cell cultures. This purified form retains the functional DNA-binding domain characteristic of SOX proteins, allowing it to interact with specific regulatory sequences in target genes. Its recombinant production facilitates studies on SOX1’s molecular mechanisms, including its role in stem cell biology, neural differentiation, and potential involvement in diseases.
Research applications of recombinant SOX1 include *in vitro* models of neurodevelopment, disease modeling, and regenerative medicine. It is also explored as a biomarker in certain cancers, as SOX1 expression has been linked to tumor suppression or oncogenesis in contexts like lung and breast cancer. Additionally, SOX1 recombinant protein is utilized in developmental biology to investigate its interplay with signaling pathways (e.g., Wnt, Notch) and other transcription factors during tissue specification.
The availability of recombinant SOX1 has advanced both basic and translational research, offering insights into its therapeutic potential for neurodegenerative disorders or cancer therapies. However, challenges remain in understanding its context-dependent roles and optimizing delivery mechanisms for clinical applications. Overall, recombinant SOX1 serves as a vital tool for dissecting the complexities of developmental biology and disease pathology.
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