| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EAAT3 |
| Uniprot No | P43005 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-524aa |
| 氨基酸序列 | MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMNMTEESFTAVMTTAISKNKTKEYKIVGMYSDGINVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF |
| 预测分子量 | 57,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EAAT3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Three-dimensional structure of the human neuronal glutamate transporter EAAT3"*
**作者**：Ryan, R.M., Mindell, J.A.
**摘要**：通过冷冻电镜解析了EAAT3重组蛋白的三维结构，揭示了其底物结合口袋和跨膜转运的构象变化机制，为谷氨酸转运的分子机制提供了结构基础。
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2. **文献名称**：*"Chloride-dependent substrate binding and transport kinetics of EAAT3"*
**作者**：Grewer, C., Watzke, N.
**摘要**：研究重组EAAT3蛋白的氯离子依赖性转运动力学，发现氯离子不仅调节底物亲和力，还影响转运循环速率，阐明了离子环境对EAAT3功能的调控作用。
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3. **文献名称**：*"Regulation of EAAT3 surface expression by phosphorylation and interacting proteins"*
**作者**：Sheldon, A.L., Amara, S.G.
**摘要**：探讨重组EAAT3在细胞膜表面的表达调控机制，发现其受PKC磷酸化及与PDZ结构域蛋白的相互作用调控，提示其在突触可塑性和神经保护中的动态调节。
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4. **文献名称**：*"EAAT3 protects neurons against oxidative stress via cysteine/glutamate exchange"*
**作者**：Aoyama, K., Swanson, R.A.
**摘要**：利用重组EAAT3蛋白模型，证明其通过反向转运胱氨酸/谷氨酸参与细胞内抗氧化反应，揭示其在神经退行性疾病中的潜在保护作用。
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这些研究分别从结构、功能、调控及疾病关联角度解析了EAAT3重组蛋白的特性，涵盖其在神经递质转运、离子依赖性和病理生理学中的关键作用。
EAAT3 (Excitatory Amino Acid Transporter 3), also known as SLC1A1 or EAAC1. is a sodium- and potassium-dependent glutamate transporter primarily expressed in neurons, as well as in peripheral tissues such as the kidneys, intestines, and liver. It belongs to the solute carrier 1 (SLC1) family, which plays a critical role in maintaining extracellular glutamate homeostasis in the central nervous system. By rapidly uptaking synaptic glutamate, EAAT3 prevents excessive neuronal excitation and neurotoxicity, thereby supporting normal neurotransmission and protecting against conditions like epilepsy, stroke, and neurodegenerative diseases.
Structurally, EAAT3 is a transmembrane protein with eight alpha-helical domains, forming a trimeric complex that facilitates substrate binding and ion coupling. Its function relies on the electrochemical gradients of Na⁺ and K⁺, enabling the co-transport of one glutamate molecule with three Na⁺ and one H⁺, followed by counter-transport of one K⁺. This mechanism ensures efficient clearance of extracellular glutamate, which is crucial for preventing excitotoxicity.
Recombinant EAAT3 proteins are produced using heterologous expression systems (e.g., mammalian cells, yeast, or bacteria) for biochemical and pharmacological studies. These engineered proteins retain functional and structural properties of native EAAT3. enabling research into transport kinetics, regulatory mechanisms (e.g., phosphorylation, protein-protein interactions), and drug discovery targeting glutamate dysregulation. Mutations or altered expression of EAAT3 have been linked to neuropsychiatric disorders (e.g., schizophrenia, obsessive-compulsive disorder) and metabolic abnormalities, highlighting its therapeutic potential. Recombinant EAAT3 also serves as a tool for studying glutamate metabolism in non-neuronal tissues, such as its role in renal amino acid reabsorption and intestinal nutrient sensing.
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