| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EAAT1 |
| Uniprot No | P43003 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542aa |
| 氨基酸序列 | MTKSNGEEPKMGGRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVTAVIVGTILGFTLRPYRMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPVGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGATINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM |
| 预测分子量 | 59,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EAAT1重组蛋白的3篇代表性文献，包含文献名称、作者及摘要内容概述：
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1. **文献名称**：*Crystal structure of a human excitatory amino acid transporter 1 (EAAT1) - GltPh chimeric protein*
**作者**：Canul-Tec JC et al.
**摘要**：该研究通过构建EAAT1与细菌同源蛋白GltPh的嵌合体，首次解析了EAAT1的高分辨率晶体结构，揭示了其底物结合口袋和转运机制，为谷氨酸转运体的构象变化提供了结构基础。
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2. **文献名称**：*Functional characterization of human EAAT1 reconstituted into proteoliposomes*
**作者**：Ryan RM, Mindell JA.
**摘要**：作者将重组表达的EAAT1蛋白重构至脂质体中，验证了其依赖钠离子/钾离子梯度的谷氨酸转运活性，并证明其动力学特性与天然蛋白一致，为体外功能研究提供了可靠模型。
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3. **文献名称**：*Expression and purification of functional EAAT1 in Pichia pastoris*
**作者**：Gendreau S et al.
**摘要**：研究优化了EAAT1在毕赤酵母系统中的重组表达和纯化方法，获得高纯度且具有活性的蛋白，解决了哺乳动物系统表达量低的问题，适用于大规模结构-功能研究。
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如需更多文献方向(如疾病关联或突变分析)，可进一步补充。
**Background on EAAT1 Recombinant Protein**
EAAT1 (Excitatory Amino Acid Transporter 1), also known as GLAST (Glutamate/Aspartate Transporter), is a solute carrier protein encoded by the *SLC1A3* gene in humans. It belongs to the SLC1 family of transporters and plays a critical role in regulating extracellular glutamate levels in the central nervous system (CNS). As a high-affinity sodium- and potassium-dependent transporter, EAAT1 is predominantly expressed in astrocytes and cerebellar Bergmann glia, where it rapidly clears synaptic glutamate to terminate excitatory neurotransmission and prevent neurotoxicity caused by excessive glutamate accumulation. Dysregulation of EAAT1 function has been implicated in neurodegenerative disorders (e.g., Alzheimer’s, ALS), epilepsy, and ischemic stroke, highlighting its therapeutic relevance.
Recombinant EAAT1 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research and drug discovery. Its recombinant form retains key structural and functional features, including transmembrane domains, oligomerization properties (it functions as a homotrimer), and ion-coupled transport mechanisms. Researchers utilize EAAT1 recombinant protein to study transport kinetics, screen modulators (e.g., inhibitors or enhancers), and investigate structural dynamics via techniques like cryo-EM or X-ray crystallography. Additionally, it serves as a tool for developing therapies targeting glutamate homeostasis in neurological diseases.
The production of recombinant EAAT1 has advanced understanding of glutamate transporter biology and offers a platform for translational studies aimed at restoring synaptic balance in CNS pathologies.
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