| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAM2 |
| Uniprot No | O75886 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-525aa |
| 氨基酸序列 | MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAI MKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNK AHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQT VSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLN NKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGL FPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRA LQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSEL NVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSH GGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLS TGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSYQNTTSNLPQL AGFPVTVPAHPVAQQHTNYHQQPLL |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于STAM2重组蛋白的相关文献(信息基于公开论文数据整合，非真实文献，仅供参考)：
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1. **文献名称**：*Structural and Functional Analysis of STAM2 in EGFR Signaling*
**作者**：T. Kobayashi et al.
**摘要**：通过在大肠杆菌中表达重组STAM2蛋白(含GST标签)，解析其VHS结构域与泛素化EGFR的相互作用，揭示STAM2在内吞排序中的作用机制。
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2. **文献名称**：*Recombinant STAM2 Facilitates ESCRT-III Assembly in Autophagy*
**作者**：L. Chen & M. Nakayama
**摘要**：利用昆虫细胞系统表达重组人源STAM2蛋白，通过体外结合实验证明其UBD结构域招募ESCRT-III组分，调控自噬溶酶体成熟过程。
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3. **文献名称**：*Kinetic Study of STAM2-Hrs Complex Using Surface Plasmon Resonance*
**作者**：R. Gupta et al.
**摘要**：基于哺乳细胞表达纯化的His-STAM2重组蛋白，利用SPR技术定量分析其与Hrs蛋白的亲和力，揭示生长因子信号传导的动态调控机制。
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注：若需真实文献，建议在PubMed中以“STAM2 recombinant protein”或“STAM2 expression”为关键词检索近年研究。
**Background of STAM2 Recombinant Protein**
STAM2 (Signal Transducing Adaptor Molecule 2) is a cytosolic protein involved in intracellular signal transduction and membrane trafficking. It belongs to the STAM family, which includes STAM1 and STAM2. both sharing conserved domains but exhibiting tissue-specific expression and functional nuances. STAM2 plays a critical role in regulating growth factor and cytokine receptor signaling pathways, such as those mediated by epidermal growth factor (EGF) and interleukin-2 (IL-2).
Structurally, STAM2 contains a VHS (Vps27. Hrs, STAM) domain, an SH3 (Src homology 3) domain, and an ITAM (Immunoreceptor Tyrosine-based Activation Motif). These domains facilitate interactions with ubiquitinated proteins, endosomal sorting complexes, and signaling molecules. Notably, STAM2 collaborates with Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) to form the ESCRT-0 complex, which sorts ubiquitinated cargo proteins into multivesicular bodies (MVBs) for lysosomal degradation. This process is vital for terminating receptor signaling and maintaining cellular homeostasis.
Recombinant STAM2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional STAM2 for research. It often includes affinity tags (e.g., GST, His-tag) for easy purification and detection. Studies utilizing recombinant STAM2 have elucidated its role in endosomal sorting, signal modulation, and cross-talk with pathways like PI3K/Akt and MAPK. Dysregulation of STAM2 is linked to cancers, immune disorders, and neurodegenerative diseases, highlighting its therapeutic potential.
In experimental settings, recombinant STAM2 enables *in vitro* binding assays, structural studies, and screening for inhibitors targeting its interaction domains. Its application advances understanding of cellular trafficking mechanisms and disease-associated signaling anomalies.
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