| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAM1 |
| Uniprot No | P70297 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-548aa |
| 氨基酸序列 | PLFATNPFDQDVEKATSELNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHV AMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKN DPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVATKKEEEDLAKAIE LSLKEQRQQSAPVSTLYPSTSNLLTNHQHEGRKVRAVYDFEAAEDNELTFKAGEIITVLD DSDPNWWKGETHQGVGLFPSNFVTADLTAEPEMIKTEKKTVQFNDDVQIETIEPEPEPAF IDEDKMDQLLQMLQSTDPSDNQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELN VKVMEALSLYTKLMNEDPMYSMYAKLQSQQYYLQSSAVSASQVYPGPAQSGTYLVAGSAQ MTHLQSYSLPPEQLSSISQGAVPSSANQALPSQQTQASYPNAMVSSVQGNSYPSQASIYS PPAAAAAAAAAAVVPVPVPADVTIYQNAGPTMSQVPNYTLTSSTLPQTGGSQQPPQPQQA YSQKALL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAM1重组蛋白的3-4篇参考文献及其简要摘要：
1. **"Hrs recruits clathrin to early endosomes"**
- **作者**: Takeshi Kurihara 等
- **摘要**: 该研究通过重组蛋白技术揭示了STAM1与Hrs蛋白在内体分选中的相互作用，调控EGF受体的降解过程，阐明了STAM1在生长因子信号传导中的适配功能。
2. **"The Hrs-STAM complex acts as a coordination hub for signaling ubiquitination"**
- **作者**: Kirsten Bache 等
- **摘要**: 利用重组STAM1的VHS结构域进行结构分析，发现其与泛素化修饰的相互作用，揭示了STAM1在泛素依赖性膜运输中的分子机制。
3. **"STAM1 is essential for T-cell receptor signaling"**
- **作者**: Makoto Yamada 等
- **摘要**: 通过重组蛋白实验，证明STAM1参与Toll样受体(TLR)信号通路，调控免疫反应中的细胞因子分泌，强调了其在先天免疫中的适配作用。
4. **"AMSH interacts with STAM through the MIT domain"**
- **作者**: Mineko Kato 等
- **摘要**: 研究使用重组STAM1蛋白与AMSH进行体外泛素化实验，揭示了二者通过MIT结构域结合，调控受体酪氨酸激酶的内体分选过程。
这些文献均涉及重组蛋白技术在STAM1功能研究中的应用，涵盖信号传导、泛素化调控及免疫反应等领域。
**Background of STAM1 Recombinant Protein**
STAM1 (Signal Transducing Adaptor Molecule 1) is a cytoplasmic adaptor protein involved in intracellular signal transduction and membrane trafficking. It was initially identified as a component of the EGF (epidermal growth factor) receptor signaling pathway, where it interacts with other adaptors like Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) to regulate endosomal sorting and ubiquitin-dependent protein sorting. Structurally, STAM1 contains an SH3 domain, an ITAM (Immunoreceptor Tyrosine-Based Activation Motif), and a VHS (Vps27. Hrs, STAM) domain, enabling interactions with ubiquitinated cargo proteins and signaling molecules.
Recombinant STAM1 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional STAM1 for research. This allows scientists to study its role in cytokine signaling (e.g., IL-2. GM-CSF), receptor endocytosis, and lysosomal degradation pathways. STAM1 is critical for cell proliferation, immune responses, and receptor downregulation, with dysregulation linked to cancers and immune disorders.
Researchers utilize recombinant STAM1 to investigate its binding partners, post-translational modifications (e.g., tyrosine phosphorylation), and its crosstalk with STAM2. a homologous protein. These studies provide insights into diseases like leukemia or immunodeficiency syndromes and aid in developing targeted therapies. The recombinant protein is also essential for in vitro assays, structural studies, and screening molecules that modulate STAM1-dependent pathways. Overall, STAM1 recombinant protein serves as a vital tool for decoding cellular signaling networks and their pathological implications.
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