| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA3E |
| Uniprot No | O15041 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-775aa |
| 氨基酸序列 | ADTTH PRLRLSHKEL LNLNRTSIFH SPFGFLDLHT MLLDEYQERL FVGGRDLVYS LSLERISDGY KEIHWPSTAL KMEECIMKGK DAGECANYVR VLHHYNRTHL LTCGTGAFDP VCAFIRVGYH LEDPLFHLES PRSERGRGRC PFDPSSSFIS TLIGSELFAG LYSDYWSRDA AIFRSMGRLA HIRTEHDDER LLKEPKFVGS YMIPDNEDRD DNKVYFFFTE KALEAENNAH AIYTRVGRLC VNDVGGQRIL VNKWSTFLKA RLVCSVPGMN GIDTYFDELE DVFLLPTRDH KNPVIFGLFN TTSNIFRGHA ICVYHMSSIR AAFNGPYAHK EGPEYHWSVY EGKVPYPRPG SCASKVNGGR YGTTKDYPDD AIRFARSHPL MYQAIKPAHK KPILVKTDGK YNLKQIAVDR VEAEDGQYDV LFIGTDNGIV LKVITIYNQE MESMEEVILE ELQIFKDPVP IISMEISSKR QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD GISCSRYYPT GTHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDKTEEHLA YGIENNSTLL ECTPRSLQAK VIWFVQKGRE TRKEEVKTDD RVVKMDLGLL FLRLHKSDAG TYFCQTVEHS FVHTVRKITL EVVEEEKVED MFNKDDEEDR HHRMPCPAQS SISQGAKPWY KEFLQLIGYS NFQRVEEYCE KVWCTDRKRK KLKMSPSKWK YANPQEKKLR SKPEHYRLPR HTLDS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA3E重组蛋白的3篇参考文献概览：
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1. **文献名称**：*SEMA3E suppresses tumor cell death through Plexin D1/Akt-dependent mTOR pathway*
**作者**：Casazza A, et al. (2013)
**摘要**：该研究发现SEMA3E重组蛋白通过结合受体Plexin D1激活Akt/mTOR信号通路，抑制肿瘤细胞凋亡，并促进肿瘤血管异常生成，揭示了其在癌症进展中的双重作用。
2. **文献名称**：*SEMA3E regulates endothelial to mesenchymal transition through control of integrin activity*
**作者**：Kim J, et al. (2018)
**摘要**：研究表明，重组SEMA3E通过调控整合素活性抑制内皮-间质转化(EndMT)，减少病理性血管重塑，为治疗心血管疾病提供了潜在靶点。
3. **文献名称**：*SEMA3E-Plexin D1 signaling modulates cancer cell invasiveness and metabolic reprogramming*
**作者**：Sakurai A, et al. (2020)
**摘要**：文章揭示SEMA3E重组蛋白通过Plexin D1受体增强癌细胞侵袭能力，并诱导糖酵解代谢重编程，提示其在肿瘤转移中的关键调控作用。
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以上研究分别从肿瘤存活、血管重塑及代谢调控角度阐明了SEMA3E重组蛋白的功能机制。如需具体文献链接或补充其他方向研究，可进一步说明。
**Background of SEMA3E Recombinant Protein**
SEMA3E, a member of the class 3 semaphorin family, is a secreted glycoprotein known for its role in regulating neuronal guidance, vascular development, and immune modulation. It functions as a chemorepellent or chemoattractant, depending on cellular context, by binding to receptors such as plexin-D1 and neuropilin-1. SEMA3E is critical during embryogenesis, particularly in axon pathfinding, neural crest cell migration, and cardiovascular patterning. Dysregulation of SEMA3E has been implicated in pathological conditions, including cancer metastasis, angiogenesis-related disorders, and neurological diseases.
The recombinant SEMA3E protein is engineered using mammalian or bacterial expression systems to ensure proper post-translational modifications and bioactivity. It typically retains the conserved SEMA domain, essential for receptor interaction, and is purified via affinity chromatography. Researchers utilize SEMA3E recombinant protein in vitro and in vivo to study its signaling mechanisms, such as its ability to inhibit integrin-mediated cell adhesion or modulate MAPK/ERK pathways. In cancer biology, SEMA3E exhibits dual roles—suppressing tumor growth by normalizing vasculature or promoting invasion via EMT (epithelial-mesenchymal transition).
Recent studies also explore its therapeutic potential. For example, SEMA3E-based strategies are being tested to target tumor microenvironments or repair neural circuits. Its involvement in immune cell trafficking further broadens its relevance in inflammatory diseases. However, context-dependent effects necessitate careful evaluation in therapeutic applications. Overall, SEMA3E recombinant protein serves as a vital tool for dissecting cellular communication networks and developing interventions for complex diseases.
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