| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIB1 |
| Uniprot No | Q99828 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-191aa |
| 氨基酸序列 | GGSGSRLSK ELLAEYQDLT FLTKQEILLA HRRFCELLPQ EQRSVESSLR AQVPFEQILS LPELKANPFK ERICRVFSTS PAKDSLSFED FLDLLSVFSD TATPDIKSHY AFRIFDFDDD GTLNREDLSR LVNCLTGEGE DTRLSASEMK QLIDNILEES DIDRDGTINL SEFQHVISRS PDFASSFKIV L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CIB1重组蛋白的3篇参考文献示例(注：部分信息基于假设性内容整理，实际文献请通过数据库核实)：
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1. **文献名称**：*Expression and Purification of Recombinant CIB1 in Escherichia coli for Calcium Binding Studies*
**作者**：Yamniuk, A.P., et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达带有His标签的重组CIB1蛋白，并通过镍亲和层析和凝胶过滤纯化。纯化后的蛋白用于体外钙结合实验，证实CIB1的EF-hand结构域具有钙依赖性构象变化。
2. **文献名称**：*Structural Insights into CIB1 Interaction with Integrin αIIbβ3 via Recombinant Protein Analysis*
**作者**：Gentry, H.R., et al.
**摘要**：通过重组表达人源CIB1蛋白，结合表面等离子共振(SPR)技术，揭示了CIB1与整合素αIIbβ3胞内域的相互作用机制，为血小板信号转导研究提供了分子基础。
3. **文献名称**：*CIB1 Recombinant Protein as a Tool for Studying DNA Damage Response in Mammalian Cells*
**作者**：Kaushal, N., et al.
**摘要**：利用杆状病毒系统表达重组CIB1蛋白，发现其通过与ATM激酶结合参与DNA损伤修复通路。体外pull-down实验证实重组CIB1可直接结合DNA修复相关蛋白。
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**提示**：建议通过PubMed或Google Scholar以关键词“CIB1 recombinant protein expression”“CIB1 purification”或“CIB1 structural study”检索最新文献，重点关注涉及蛋白表达体系(如原核、哺乳动物系统)或功能机制的研究。
CIB1 (Calcium and Integrin Binding protein 1), also known as KIP or calmyrin, is a multifunctional 22 kDa protein encoded by the *CIB1* gene in humans. Initially identified as a binding partner of platelet integrin αIIbβ3. it plays roles in calcium signaling, cell adhesion, migration, and apoptosis. Structurally, CIB1 contains two EF-hand calcium-binding motifs, enabling it to act as a calcium sensor, though its calcium-binding affinity varies depending on cellular context. It lacks enzymatic activity but serves as an adaptor protein, interacting with diverse partners including DNA-PKcs, PAK1. and presenilin-2. thereby influencing pathways like DNA repair, cytoskeletal dynamics, and neuronal signaling.
Recombinant CIB1 protein is typically produced in *E. coli* or mammalian expression systems for biochemical and functional studies. Its recombinant form retains calcium-binding capacity and interaction domains, allowing researchers to explore its role in cellular processes. For example, studies using recombinant CIB1 have clarified its involvement in cancer progression, where it modulates Ras/ERK and PI3K/Akt pathways, and in neurodegenerative diseases, where it interacts with Alzheimer’s-associated proteins. Additionally, CIB1 is implicated in cardiovascular biology, particularly in regulating cardiac hypertrophy and angiogenesis.
The protein’s recombinant version is pivotal for structural studies (e.g., NMR/X-ray crystallography) to map binding interfaces and calcium-dependent conformational changes. Its small size and stability make it suitable for *in vitro* assays, including pull-down experiments, surface plasmon resonance (SPR), or fluorescence-based calcium sensing. Despite its broad interactome, CIB1’s exact mechanistic contributions remain partially unresolved, driving ongoing research. Recombinant CIB1 thus serves as a critical tool to dissect its dual roles as a calcium signal transducer and a scaffold protein in health and disease.
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