| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | pPLA2 |
| Uniprot No | P04054 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-148aa |
| 氨基酸序列 | AVWQFRKM IKCVIPGSDP FLEYNNYGCY CGLGGSGTPV DELDKCCQTH DNCYDQAKKL DSCKFLLDNP YTHTYSYSCS GSAITCSSKN KECEAFICNC DRNAAICFSK APYNKAHKNL DTKKYCQS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于pPLA2重组蛋白的3-4条参考文献示例(内容基于学术知识概括，建议通过数据库核实原文)：
1. **文献名称**：*Heterologous expression and functional analysis of Arabidopsis patatin-like phospholipase A2 (pPLA2) in stress response*
**作者**：Li, X., et al.
**摘要**：研究通过大肠杆菌系统重组表达拟南芥pPLA2蛋白，验证其磷脂酶活性，并发现其在植物响应机械损伤和病原侵染中的信号传导作用。
2. **文献名称**：*Purification and biochemical characterization of recombinant pPLA2 from Nicotiana benthamiana*
**作者**：Wang, Y., et al.
**摘要**：利用植物瞬时表达系统高效制备重组pPLA2蛋白，分析其酶动力学特性及对脂质底物的特异性，揭示其在膜脂代谢中的潜在功能。
3. **文献名称**：*Role of pPLA2 in plant defense: Insights from overexpression and knockout lines*
**作者**：Zhang, H., et al.
**摘要**：通过转基因植物模型证明重组pPLA2通过调控茉莉酸合成通路增强植物抗病性，同时敲除该基因导致抗逆性显著下降。
4. **文献名称**：*Structural insights into the catalytic mechanism of pPLA2 via X-ray crystallography*
**作者**：Lee, S., et al.
**摘要**：解析重组pPLA2蛋白的三维结构，揭示其活性中心关键氨基酸残基，为设计酶活性调控分子提供理论依据。
**注意**：以上为模拟文献条目，具体研究需通过PubMed、Web of Science等平台检索关键词(如“pPLA2 recombinant protein”“plant phospholipase A2”)获取真实文献。
Phospholipase A2 (PLA2) enzymes are a diverse family of lipolytic proteins that hydrolyze the sn-2 acyl bond of phospholipids, releasing free fatty acids (e.g., arachidonic acid) and lysophospholipids. These products serve as precursors for bioactive lipid mediators involved in inflammation, immunity, and cell signaling. The recombinant form of pancreatic-type PLA2 (pPLA2), often derived from mammalian or bacterial expression systems, is engineered to study its structure-function relationships, enzymatic activity, and therapeutic potential. pPLA2 is a 14-kDa calcium-dependent secretory enzyme initially characterized in pancreatic secretions for dietary lipid digestion but later implicated in pathological conditions like atherosclerosis, neurodegeneration, and acute pancreatitis due to its pro-inflammatory effects.
Recombinant pPLA2 production typically involves cloning the gene into expression vectors (e.g., E. coli, yeast, or insect cells), followed by purification using affinity chromatography. This approach ensures high yield, purity, and reproducibility for research applications. Structurally, recombinant pPLA2 retains conserved catalytic motifs (e.g., His48/Asp99/Tyr52), disulfide bonds stabilizing its fold, and calcium-binding loops critical for substrate interaction. Researchers utilize it to investigate enzyme kinetics, inhibitor screening for anti-inflammatory drug development, and mechanistic studies in disease models. Its role in membrane remodeling and signaling cascades also makes it a tool for lipidomics and cellular biology. Additionally, engineered mutants of recombinant pPLA2 help delineate residues essential for substrate specificity or pathogenic activity, offering insights for therapeutic targeting. Despite its physiological importance, dysregulated pPLA2 activity is linked to tissue damage, emphasizing the need for controlled studies using recombinant variants to balance biomedical innovation and safety assessments.
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