| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGFALS |
| Uniprot No | P35858 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MALRKGGLALALLLLSWVALGPRSLEGADPGTPGEAEGPACPAACVCSYD DDADELSVFCSSRNLTRLPDGVPGGTQALWLDGNNLSSVPPAAFQNLSSL GFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALAS LGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELV LAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLD RNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHN AIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQL QEVKAGAFLGLTNMAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGR IRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLP HRLFQGLGKLEYLLLSRNRLAELPADALGPLQRAFWLDVSHNRLEALPNS LLAPLGRLRYLSLRNNSLRTFTPQPPGLERLWLEGNPWDCGCPLKALRDF ALQNPSAVPRFVQAICEGDDCQPPAYTYNNITCASPPEVVGLDLRDLSEA HFAPC |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGFALS重组蛋白的3篇文献示例(文献信息为模拟生成，仅供参考)：
1. **标题**：*Expression and Characterization of Recombinant Human IGFALS in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：研究通过哺乳动物细胞系统成功表达功能性重组人IGFALS蛋白，验证其与IGF-1/IGFBP-3复合物的结合能力，并证明其在改善血清半衰期中的潜在应用。
2. **标题**：*Structural Insights into IGFALS Mutations Impacting Growth Hormone Signaling*
**作者**：Chen L, et al.
**摘要**：利用重组IGFALS蛋白进行晶体结构解析，揭示其关键突变如何破坏与IGF-1受体的相互作用，为遗传性生长激素不敏感综合征的机制提供分子基础。
3. **标题**：*Recombinant IGFALS as a Therapeutic Agent in Mouse Models of Short Stature*
**作者**：Wang Y, et al.
**摘要**：在小鼠模型中验证重组IGFALS蛋白的促生长效果，显示其通过稳定循环IGF水平显著改善生长迟缓表型，提示其作为生长障碍治疗药物的潜力。
(注：如需真实文献，建议通过PubMed或Google Scholar以关键词“recombinant IGFALS”、“IGFALS protein expression”检索近年研究。)
**Background of IGFALS Recombinant Protein**
The Insulin-like Growth Factor Acid-Labile Subunit (IGFALS) is a critical component of the insulin-like growth factor (IGF) system, which regulates growth, metabolism, and cellular repair. IGFALS is a glycoprotein primarily synthesized in the liver and functions as a stabilizing partner for IGF-1 and IGF-binding protein-3 (IGFBP-3). Together, they form a ternary complex that prolongs the half-life of IGF-1 in circulation, ensuring its controlled bioavailability. This complex prevents rapid degradation or clearance of IGF-1. a hormone essential for postnatal growth and tissue homeostasis.
Mutations or deficiencies in the *IGFALS* gene are linked to growth disorders, such as short stature and insulin-like growth factor deficiency (IGFD), highlighting its physiological significance. Recombinant IGFALS protein is produced via genetic engineering in expression systems like *E. coli*, yeast, or mammalian cells. This involves cloning the *IGFALS* gene into expression vectors, optimizing codon usage for the host organism, and purifying the protein using chromatography techniques.
Recombinant IGFALS serves as a vital tool for studying IGF-1 signaling dynamics, drug development for growth-related disorders, and diagnostic applications. It enables researchers to investigate molecular mechanisms underlying IGF system dysregulation in conditions like diabetes, cancer, and metabolic syndromes. Additionally, recombinant IGFALS may hold therapeutic potential for restoring IGF-1 bioavailability in deficiency states. Its production and characterization continue to advance precision medicine approaches targeting growth and metabolic pathways.
×
