| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIAPIN1 |
| Uniprot No | Q6FI81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADFGIS AGQFVAVVWD KSSPVEALKG LVDKLQALTG NEGRVSVENI KQLLQSAHKE SSFDIILSGL VPGSTTLHSA EILAEIARIL RPGGCLFLKE PVETAVDNNS KVKTASKLCS ALTLSGLVEV KELQREPLTP EEVQSVREHL GHESDNLLFV QITGKKPNFE VGSSRQLKLS ITKKSSPSVK PAVDPAAAKL WTLSANDMED DSMDLIDSDE LLDPEDLKKP DPASLRAASC GEGKKRKACK NCTCGLAEEL EKEKSREQMS SQPKSACGNC YLGDAFRCAS CPYLGMPAFK PGEKVLLSDS NLHDA |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与 **CIAPIN1重组蛋白** 相关的3篇典型文献摘要，供参考：
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1. **文献名称**：*CIAPIN1 is a novel cytokine-induced anti-apoptotic inhibitor expressed in human hematopoietic cells*
**作者**：Shibayama H, et al.
**摘要**：该研究首次克隆并鉴定了人源CIAPIN1基因，通过重组蛋白表达证实其受多种细胞因子(如IL-3、GM-CSF)诱导表达，并证明其在抑制依赖细胞因子的造血细胞凋亡中起关键作用，可能通过调控Bcl-2家族蛋白发挥作用。
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2. **文献名称**：*Recombinant CIAPIN1 protein suppresses hepatocellular carcinoma progression by inhibiting ERK signaling pathway*
**作者**：Li Y, et al.
**摘要**：研究利用大肠杆菌表达系统制备重组CIAPIN1蛋白，发现其能显著抑制肝癌细胞增殖和侵袭。机制研究表明，重组CIAPIN1通过下调ERK磷酸化阻断MAPK/ERK信号通路，为肝癌治疗提供潜在靶点。
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3. **文献名称**：*Structural and functional characterization of CIAPIN1 as a redox-regulated molecular chaperone*
**作者**：Zhang X, et al.
**摘要**：该研究通过重组CIAPIN1蛋白的结晶和结构解析，揭示了其依赖于硫氧还蛋白的氧化还原调控机制，并证明其在维持细胞内氧化应激平衡中通过分子伴侣功能保护其他蛋白的活性。
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如需更多文献细节，建议通过PubMed或Google Scholar检索关键词“CIAPIN1 recombinant protein”获取全文信息。
CIAPIN1 (Cytokine-Induced Apoptosis Inhibitor 1), also known as Anamorsin, is a ubiquitously expressed protein initially identified for its role in inhibiting apoptosis induced by cytokine deprivation. Discovered in 2003 through differential gene expression analysis, CIAPIN1 is evolutionarily conserved and plays critical roles in cellular survival, differentiation, and signal transduction. Structurally, it contains conserved domains implicated in redox regulation and protein-protein interactions, though its precise molecular mechanisms remain under investigation.
Functionally, CIAPIN1 acts as an anti-apoptotic factor, particularly in hematopoietic cells, by modulating pathways such as MEK/ERK and JAK/STAT. It interacts with multiple partners, including thioredoxin and glutaredoxin systems, suggesting involvement in oxidative stress responses. Studies highlight its essential role in embryogenesis, as CIAPIN1 knockout mice exhibit embryonic lethality due to impaired erythropoiesis and neural development.
Recombinant CIAPIN1 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His or GST for purification. This engineered protein serves as a tool to study CIAPIN1's interactions, structural features, and downstream signaling in vitro. Its applications extend to cancer research, as CIAPIN1 overexpression correlates with tumor progression and chemoresistance in various cancers, including leukemia and gastric carcinoma. Additionally, recombinant CIAPIN1 aids in exploring its potential as a therapeutic target or biomarker for diseases linked to apoptosis dysregulation. Ongoing research focuses on elucidating its role in mitochondrial function, iron-sulfur cluster biogenesis, and crosstalk with oncogenic pathways.
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