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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INSL6 |
| Uniprot No | Q9Y581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MPRLLRLSLL WLGLLLVRFS RELSDISSAR KLCGRYLVKE IEKLCGHANW SQFRFEEETP FSRLIAQASE KVEAYSPYQF ESPQTASPAR GRGTNPVSTS WEEAVNSWEM QSLPEYKDKK GYSPLGKTRE FSSSHNINVY IHENAKFQKK RRNKIKTLSN LFWGHHPQRK RRGYSEKCCL TGCTKEELSI ACLPYIDF |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INSL6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant human INSL6 exhibits distinct structural and functional properties among the insulin-like peptide family"*
**作者**: Smith A, et al.
**摘要**: 该研究利用大肠杆菌系统成功表达并纯化了重组人INSL6蛋白，发现其三维结构与胰岛素类似但具有独特的受体结合特性。实验表明INSL6在体外可激活特定G蛋白偶联受体，提示其在睾丸发育中的潜在调控作用。
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2. **文献名称**: *"Expression and characterization of mouse INSL6 recombinant protein for spermatogenesis studies"*
**作者**: Chen L, et al.
**摘要**: 通过哺乳动物细胞表达系统制备小鼠INSL6重组蛋白，并验证其生物活性。研究发现INSL6在精子形成过程中与生殖细胞表面受体相互作用，可能通过调控细胞黏附通路影响精子成熟。
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3. **文献名称**: *"Functional analysis of INSL6 mutations using recombinant protein models in cryptorchidism"*
**作者**: Tanaka K, et al.
**摘要**: 构建多种INSL6突变体重组蛋白，结合细胞实验发现特定结构域(如B链)的氨基酸突变会显著削弱其与RXFP2受体的结合能力，为隐睾症相关基因变异提供了分子机制解释。
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注：以上文献信息为示例性概括，实际文献需通过PubMed或Web of Science等数据库检索确认。若需具体文章，建议使用关键词“INSL6 recombinant protein”或“INSL6 gene function”进一步筛选。
**Background of INSL6 Recombinant Protein**
INSL6 (Insulin-like 6) is a member of the insulin/relaxin peptide family, characterized by structural homology to insulin and roles in diverse physiological processes. It shares a conserved architecture with other family members, including two peptide chains (A and B) linked by disulfide bonds, which stabilize its tertiary structure. INSL6 is primarily expressed in the testes, suggesting a potential role in male reproductive biology, though its precise functions remain less defined compared to well-studied relatives like INSL3 or INSL5.
Research indicates that INSL6 interacts with the relaxin family peptide receptor 2 (RXFP2), a G protein-coupled receptor also implicated in testicular descent and germ cell maturation. While INSL3-RXFP2 signaling is critical for gubernacular ligament development during fetal testicular descent, INSL6’s interaction with RXFP2 may influence post-natal reproductive functions, such as spermatogenesis or Leydig cell regulation. However, mechanistic insights remain limited, partly due to the challenges in studying endogenous INSL6 expression and activity.
Recombinant INSL6 protein, produced via expression systems like *E. coli* or mammalian cells, enables researchers to bypass these limitations. Its production typically involves cloning the *INSL6* gene into expression vectors, followed by purification using affinity chromatography. The recombinant protein retains structural and functional integrity, allowing in vitro and in vivo studies to explore its binding affinity, receptor activation, and downstream signaling pathways.
Current applications of INSL6 recombinant protein include elucidating its role in reproductive disorders, infertility, and potential links to cancers (e.g., testicular or ovarian). Additionally, it serves as a tool for developing diagnostic assays or therapeutic agents targeting the relaxin receptor family. Despite progress, further research is needed to clarify INSL6’s physiological relevance and therapeutic potential, highlighting the importance of recombinant protein technology in advancing this field.
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