| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRS2 |
| Uniprot No | Q9Y4H2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1338aa |
| 氨基酸序列 | MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHLKEATIVKE |
| 预测分子量 | 137,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IRS2重组蛋白的3篇参考文献示例(注：部分信息为示例性内容，实际引用时请核对原文)：
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1. **标题**: "Expression and Functional Analysis of Recombinant IRS2 in Insulin Signaling"
**作者**: Smith A, et al.
**摘要**: 研究报道了在大肠杆菌系统中成功表达并纯化IRS2重组蛋白，证实其能够有效介导胰岛素受体下游的PI3K/Akt信号通路激活，为体外研究胰岛素抵抗机制提供了工具。
2. **标题**: "IRS2 Recombinant Protein Enhances Beta-Cell Survival in Type 2 Diabetes Models"
**作者**: Johnson R, et al.
**摘要**: 通过体外实验证明，重组IRS2蛋白处理可显著抑制高糖诱导的胰岛β细胞凋亡，并促进其增殖，提示IRS2在糖尿病治疗中的潜在应用价值。
3. **标题**: "Structural Insights into IRS2 Phosphorylation via Recombinant Protein Crystallography"
**作者**: Lee S, et al.
**摘要**: 利用重组IRS2蛋白的晶体结构分析，揭示了其酪氨酸磷酸化位点的构象变化，阐明了IRS2与胰岛素受体相互作用的分子机制。
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**注意**：以上文献信息为示例，实际研究中请通过PubMed、Web of Science等学术平台检索最新文献，并确保引用准确性。如需具体文献推荐，可提供更详细的研究方向。
IRS2 (Insulin Receptor Substrate 2) is a cytoplasmic adaptor protein critical in mediating signaling pathways initiated by insulin, insulin-like growth factors (IGFs), and certain cytokines. As a member of the IRS protein family, it contains multiple tyrosine phosphorylation sites that recruit downstream effectors, such as PI3K and Grb2. to activate key cascades like the PI3K-AKT and MAPK pathways. These pathways regulate glucose homeostasis, cell growth, survival, and metabolism. IRS2 is particularly vital in pancreatic β-cell function, hepatic insulin signaling, and neuronal survival, distinguishing it from IRS1. which is more prominent in skeletal muscle and adipose tissue.
Dysregulation of IRS2 is linked to metabolic disorders, including type 2 diabetes, obesity, and insulin resistance. Its role in β-cell compensation during insulin resistance highlights its importance in delaying diabetes progression. Additionally, IRS2 has been implicated in cancer (e.g., promoting tumor cell proliferation) and neurodegenerative diseases (e.g., Alzheimer’s), underscoring its multifaceted biological impact.
Recombinant IRS2 protein is engineered for research to study these mechanisms *in vitro* or *in vivo*. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant form often includes tags (e.g., His, GST) for purification and detection. Researchers use it to investigate post-translational modifications, protein-protein interactions, and pathway dysregulation in disease models. Structural domains, such as the pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains, are preserved in recombinant IRS2 to maintain functional integrity. Its application spans drug discovery, biomarker studies, and therapeutic target validation, making it a key tool in metabolic and oncological research. However, challenges remain in mimicking native post-translational modifications, necessitating careful selection of expression systems for specific experimental goals.
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