| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CAT2 |
| Uniprot No | P25819 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MDPYKYRPASSYNSPFFTTNSGAPVWNNNSSMTVGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLNMFTFLFDDIGIPQDYRHMDGSGVNTYMLINKAGKAHYVKFHWKPTCGVKSLLEEDAIRVGGTNHSHATQDLYDSIAAGNYPEWKLFIQIIDPADEDKFDFDPLDVTKTWPEDILPLQPVGRMVLNKNIDNFFAENEQLAFCPAIIVPGIHYSDDKLLQTRVFSYADTQRHRLGPNYLQLPVNAPKCAHHNNHHEGFMNFMHRDEEVNYFPSRYDQVRHAEKYPTPPAVCSGKRERCIIEKENNFKEPGERYRTFTPERQERFIQRWIDALSDPRITHEIRSIWISYWSQADKSLGQKLASRLNVRPSI |
| 预测分子量 | 72.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAT2(阳离子氨基酸转运蛋白2)重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
---
1. **文献名称**: *"Cloning and Functional Expression of the CAT2 Cationic Amino Acid Transporter"*
**作者**: Closs, E.I., et al.
**摘要**: 该研究首次报道了CAT2转运蛋白的基因克隆及在哺乳动物细胞中的重组表达，验证了其对精氨酸等阳离子氨基酸的转运功能，并分析其在不同组织中的表达差异。
2. **文献名称**: *"Regulation of CAT2-mediated Arginine Transport in Immune Cells"*
**作者**: Hatzoglou, M., et al.
**摘要**: 研究探讨了CAT2重组蛋白在炎症和免疫反应中的作用，发现其表达受细胞因子(如TNF-α)调控，并通过调节一氧化氮(NO)合成影响巨噬细胞功能。
3. **文献名称**: *"Structural Insights into CAT2 Transport Mechanism via Recombinant Protein Mutagenesis"*
**作者**: White, M.F., García-Pérez, A.
**摘要**: 通过重组CAT2蛋白的定点突变实验，揭示了其跨膜结构域中关键氨基酸残基对底物识别和转运活性的影响，为药物靶点设计提供依据。
---
如需真实文献，建议在PubMed或Web of Science中检索关键词：“CAT2 transporter recombinant”或“SLC7A2 expression”(CAT2基因名为SLC7A2)。
CAT2 recombinant protein, derived from the cationic amino acid transporter 2 (CAT2/SLC7A2), is a key player in cellular amino acid transport, particularly for arginine, lysine, and ornithine. Belonging to the solute carrier (SLC) family, CAT2 facilitates the uptake of these cationic amino acids via a sodium-independent mechanism, supporting critical physiological processes such as nitric oxide (NO) synthesis, protein production, and immune cell function. Its expression is highly regulated under stress conditions, including hypoxia and inflammation, making it a focal point in studies related to tumor metabolism, immune regulation, and cardiovascular diseases.
Structurally, CAT2 is a transmembrane protein with 12 predicted membrane-spanning domains. It often functions as a heterodimer, requiring association with glycoproteins like 4F2hc (CD98) for proper membrane localization and activity. Recombinant CAT2 proteins are typically produced in mammalian or insect cell systems to ensure post-translational modifications and functional integrity. These proteins are purified using affinity tags (e.g., His-tag) and validated via transport assays to confirm substrate specificity.
Research applications of CAT2 recombinant protein span cancer biology—where tumor cells exploit CAT2 for arginine uptake to sustain proliferation—and immunology, as arginine metabolism modulates T-cell activation and macrophage polarization. In cardiovascular research, CAT2's role in regulating NO synthesis links it to vascular function and hypertension. Additionally, its involvement in nutrient-sensing pathways (e.g., mTOR signaling) underscores its therapeutic potential in metabolic disorders. CAT2 recombinant tools enable mechanistic studies and drug discovery targeting amino acid transport pathologies.
×
