| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTHFR |
| Uniprot No | P42898 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MVNEARGNSSLNPCLEGSASSGSESSKDSSRCSTPGLDPERHERLREKMRRRLESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIGDQWEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEAGSFEADLKHLKEKVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLELLNRPTQNARETEAP |
| 预测分子量 | 76.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MTHFR重组蛋白的模拟参考文献(基于公开研究领域常见方向，非真实文献)：
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1. **文献名称**：*Expression and Purification of Human MTHFR Recombinant Protein in E. coli*
**作者**：J. Chen et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功克隆并纯化人源MTHFR重组蛋白，优化了表达条件以提高酶活性，为后续酶动力学研究提供基础材料。
2. **文献名称**：*Functional Characterization of MTHFR C677T Polymorphism Using Recombinant Protein Assays*
**作者**：M. Rodríguez et al.
**摘要**：通过重组蛋白技术构建MTHFR C677T突变体，发现该突变导致酶热稳定性下降及叶酸代谢活性降低，揭示了其与高同型半胱氨酸血症的关联机制。
3. **文献名称**：*Crystallographic Analysis of Recombinant MTHFR Reveals Substrate Binding Sites*
**作者**：K. Tanaka & S. Patel
**摘要**：首次解析了重组MTHFR蛋白的晶体结构，明确了其与底物(5.10-亚甲基四氢叶酸)的结合位点，为靶向药物设计提供结构依据。
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**注**：以上文献为模拟内容，实际研究中建议通过PubMed、Google Scholar等平台以关键词“MTHFR recombinant protein”检索真实文献。
**Background of MTHFR Recombinant Protein**
Methylenetetrahydrofolate reductase (MTHFR) is a critical enzyme in folate metabolism, catalyzing the irreversible conversion of 5.10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. This reaction is essential for homocysteine remethylation to methionine, a process vital for DNA synthesis, methylation reactions, and nucleotide regulation. Genetic polymorphisms in *MTHFR*, particularly the C677T variant, are linked to reduced enzyme activity, altering folate and homocysteine levels. These disruptions are associated with increased risks of cardiovascular diseases, neural tube defects, and certain cancers, highlighting MTHFR's role in human health.
Recombinant MTHFR protein is produced using biotechnological systems (e.g., *E. coli*, yeast, or mammalian cells*) to study its structure, function, and interactions. This engineered protein enables researchers to bypass challenges in isolating native MTHFR from tissues, offering consistent purity and scalability. It serves as a tool to investigate the biochemical effects of genetic variants, screen potential therapeutic agents, and develop diagnostic assays for MTHFR-related disorders.
Studies using recombinant MTHFR have clarified mechanisms underlying enzyme dysfunction, such as thermolability in the C677T variant, and guided personalized interventions (e.g., folate supplementation). Additionally, its use in structural biology has advanced drug discovery targeting MTHFR-associated pathologies. Overall, recombinant MTHFR protein bridges genetic insights and clinical applications, fostering precision medicine approaches for folate-metabolism-related conditions.
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